丹酚酸B对兔缺血再灌注心脏内皮细胞功能和血小板活化的影响  

Effects of salvianolic acid B on cardiovascular endothelial cells and platelet activation in a rabbit model of ischemia-reperfusion

作　　者：杨富国[1] 张安勇[2] 陈作元[3] 廉哲勋[3] 刘革新[1] 董果雄[3] 

机构地区：[1]青岛大学医学院护理学院内科学教研室,山东青岛266021 [2]青岛大学医学院设备科,山东青岛266021 [3]青岛大学医学院附属医院心内科,山东青岛266003

出　　处：《中西医结合学报》2008年第12期1250-1254,共5页Journal of Chinese Integrative Medicine

摘　　要：目的:观察丹酚酸B(salvianolicacid B,SA-B)对兔缺血再灌注心脏内皮细胞功能和血小板活化的影响。方法:将24只新西兰大耳白兔随机分为假手术组、缺血再灌注损伤组(模型组)和SA-B治疗组。结扎兔心脏左前降支0.5h造成心肌缺血后再灌注4h制成心肌缺血再灌注模型。SA-B治疗组于左前降支结扎后静滴SA-B溶液,其余各组静滴等量生理盐水。分别于结扎前、缺血0.5 h、再灌注1h及4h静脉采血,用硝酸还原酶法测定血浆一氧化氮(nitric oxide,NO)浓度,放射免疫法测定血浆内皮素(endothelin,ET)含量和血小板表面α颗粒膜蛋白140(alpha-granule membrane protein-140,GMP-140)的数目。结果:假手术组手术前后血浆NO浓度、ET含量和血小板表面GMP-140差别无统计学意义(P>0.05);模型组心肌缺血0.5h后,血浆NO浓度较术前及假手术组相应时间显著降低(P<0.05),并随再灌注时间延长呈进行性下降,而血浆ET含量和血小板表面GMP-140数目较术前及假手术组相应时间显著升高(P<0.05),并随再灌注时间延长进一步上升;治疗组应用SA-B后,再灌注1h及4h时血浆NO浓度较模型组相应时间明显升高(P<0.01),而血浆ET含量和血小板表面GMP-140数目较模型组相应时间明显降低(P<0.01)。结论:心脏缺血再灌注过程中存在血管内皮细胞功能损伤及血小板活化,SA-B具有保护内皮细胞和抑制血小板活化的作用。Objective： To investigate the effects of salvianolic acid B （SA-B） on cardiovascular and platelet activation during myocardial ischemia-reperfusion in rabbits. Methods： A total of 24 New Zealand white rabbits were randomly divided into ischemia-reperfusion group （untreated group） and SA-B group. The hearts of rabbits endothelial cell function sham-operated group, in untreated group and SA-B group underwent half an hour of left anterior descending coronary artery （LADCA） occlusion via ligation technology, which was followed by 4 hours of reperfusion to prepared ischemia-reperfusion injury model in vivo. For sham-operated group, the animals were not subjected to occlusion of LADCA. In SA-B treatment group the rabbits were intravenously administered SA-B immediately after LADCA occlusion, and the other two groups were given normal saline in the same way instead of SA-B. The jugular vein bloods of animals were collected before LADCA ligation, half an hour after ligation and after 1-, 4-hour reperfusion, respectively. The content of plasma nitric oxide （NO） was determined by nitrate reductase process. Radioimmunoassay was applied to detect the endothelin （ET） content in plasma and the count of alphagranule membrane protein-140 （GMP-140） on platelet surface to identify the activation of the platelet. Results. No significant difference was observed before and after sham LADCA occlusion in sham-operated group in the contents of NO and ET in plasma （P〈0.05）, neither was the count of GMP-140 on platelet surface （P〉0.05）. The content of NO in plasma detected 0.5 h after LADCA occlusion was significantly decreased in untreated group compared with the sham-operated group at the corresponding time, and they were also much lower than that before LADCA occlusion in the sham-operated group （P〈0.05）. The plasma content of NO in untreated group showed a progressive decrease in response to the myocardial reperfusion. However, the content of ET in plasma and the count of GMP-140 on platel

关 键 词：丹酚酸B 心肌缺血再灌注损伤 一氧化氯 内皮素 血小板Α颗粒膜蛋白140 兔 

分 类 号：R285.5[医药卫生—中药学]