产物诱变选育鼠李糖脂高产菌株的研究  

Screening of A High-yielding Strain of Rhamnolipid by Mutation

作　　者：秦新政[1,2] 杨新平[1,2] 唐娴[1,2] 古丽尼莎·沙依木[2] 冯蕾[2] 陈竞[2] 张慧涛[1,2] 侯新强[1,2] 王博[2] 

机构地区：[1]乌鲁木齐优耐特生物技术有限公司,乌鲁木齐830000 [2]新疆农科院微生物所,乌鲁木齐830091

出　　处：《新疆农业科学》2008年第6期1060-1063,共4页Xinjiang Agricultural Sciences

基　　金：乌鲁木齐市科技局基金项目”采油用生物表面活性剂的发酵工艺研究”(K06113010)

摘　　要：对鼠李糖脂生产菌种假单胞菌Pseudomonas sp. sy3进行自身产物诱变选育高产菌株,并对生产鼠李糖脂的培养基和发酵条件进行了研究。经过鼠李糖脂产物诱变,获得1株鼠李糖脂产量较高的正突变株322,该突变菌株鼠李糖脂产量达到27.5 g/L,比出发菌株提高了57.14%。Screening of Rhamnolipid- producing Pseudomonas Mutants was carried out by rhamnolipid . The fermentation conditions in shaking flask were investigated. The optimal medium and the fermentation technology were demonstrated.After mutation of rhamnolcpid, a high - yielding strain of rharnnolipcd, namely strain 322, was obtailced,whose yield was 27.5g/L,which was 57.14% higher than that by the original strain.

关 键 词：鼠李糖脂 假单胞菌 生物表面活性剂 

分 类 号：S188[农业科学—农业基础科学]