甲基强的松龙对重症急性胰腺炎大鼠脑组织神经细胞凋亡的影响  

Effects of methylprednisolone on neurocyte apoptosis in rats with severe acute pancreatitis

作　　者：周一农 黄鹤光[1] 李赟[1] 陈先强[1] 刘夏磊[1] 

机构地区：[1]福建医科大学附属协和医院普外科,福州350001 [2]浙江省中医药大学附属衢州中心医院

出　　处：《中华消化外科杂志》2008年第6期419-421,共3页Chinese Journal of Digestive Surgery

基　　金：基金项目：福建省教育厅科研基金资助项目（JA03099）

摘　　要：目的探讨甲基强的松龙对重症急性胰腺炎大鼠脑组织神经细胞凋亡的影响。方法将36只SD大鼠分为3组，假手术组、重症急性胰腺炎组、甲基强的松龙组，每组12只。逆行胰胆管注射5％牛磺脱氧胆酸钠建立重症急性胰腺炎模型。观察各组血清淀粉酶、IL-6、TNF-α水平、腹水量和胰腺组织的病理学改变。RT-PCR法分析脑组织Bcl-2、Bax mRNA的表达水平，TUNEL法检测神经细胞凋亡。结果重症急性胰腺炎组血清IL-6、TNF-α升高，脑组织Bcl-2 mRNA表达减少，Bax mRNA表达上调，Bcl-2／Bax比值降低，脑组织神经细胞凋亡增加；甲基强的松龙组血清IL-6、TNF-α表达明显下降，脑组织Bcl-2 mRNA表达变化不明显，但Bax mRNA表达下调明显，Bcl-2／Bax比值升高，脑组织神经细胞凋亡显著减少。结论重症急性胰腺炎时脑组织神经细胞凋亡可能是胰性脑病的发病机制之一；甲基强的松龙可抑制细胞因子的释放，促进脑组织Bcl-2和Bax基因表达的平衡，降低脑组织神经细胞凋亡指数，使脑组织损伤得以改善。Objective To investigate the effects of methylprednisolone on neurocyte apoptosis in rats with severe acute pancreatitis （SAP）. Methods Thirty-six SD rats were divided into sham operation group, SAP group and methylprednisolone group （ 12 rats in each group）. SAP model was constructed by injecting 5% sodium taurodeoxycholate into biliary-panereatic duct. Serum amylase, interleukin-6 （ IL-6 ） , tumor necrosis factor α （TNF-α） , volume of ascites and histopathoiogical changes of pancreas were determined. The mRNA expressions of Bcl-2 and Bax in brain tissue were analyzed by RT-PCR, and neurocyte apoptosis was detected by TUNEL method. Results The levels of serum IL-6 and TNF-α were significantly increased; the expression of Bcl-2 mRNA in brain tissue was down-regulated; the expression of Bax mRNA was up-regulated; the Bcl-2/Bax ratio was decreased ; the apoptosis of the neurocytes was increased in SAP group. Compared with SAP group, the levels of serum IL-6 and TNF-α were significantly decreased; the expression of Bcl-2 mRNA was unchanged but the expression of Bax mRNA was down-regulated in brain tissue, so the Bcl-2/Bax ratio was elevated significantly; the rate of the neurocyte apoptosis in brain tissue were reduced in methylprednisolone group. Conclusions The apoptosis of neurocytes in brain tissue may be one of the factors causing pancreatic encephalopathy. Methylprednisolone can inhibit the release of IL-6 and TNF-α, improve the balance of Bcl-2 and Bax expression and decrease the apoptosis of neurocytes in brain tissue.

关 键 词：胰腺炎 急性 甲基强的松龙 凋亡 神经细胞 

分 类 号：R285.5[医药卫生—中药学]