猪肝肠联合移植免疫耐受的实验研究  

Liver graft induces the immune tolerance of intestinal allograft in auxiliary en-bloc liver-small bowel transplantation in pigs

作　　者：尹震宇[2] 王效民[3] 李宁[1] 李幼生[1] 倪小冬[1] 蒋峰[1] 黎介寿[1] 

机构地区：[1]南京军区南京总医院全军普通外科研究所,210093 [2]厦门大学附属中山医院肝胆外科 [3]厦门大学附属中山医院肝胆外科,361004

出　　处：《中华消化外科杂志》2008年第6期436-438,共3页Chinese Journal of Digestive Surgery

基　　金：基金项目：国家自然科学基金资助项目（39070828）;厦门市卫生局重点资助项目（2006）

摘　　要：目的研究猪肝肠联合移植中肝移植物对同源小肠移植物免疫耐受的作用。方法70头杂交长白猪分为4组，A、B、C组为辅助性同种异体肝肠联合移植（每组20头）；D组为节段性同种异体小肠移植（10头）。移植后A、D组未用免疫抑制剂治疗，B、C组分别采用常规剂量和小剂量的环孢素和甲基强的松龙治疗。结果A组术后小肠移植物较D组排斥反应时间延迟，程度明显减轻（P〈0．05）。常规剂量的B组与小剂量的C组在术后存活时间、排斥反应开始时间以及排斥反应程度方面差异无统计学意义（P〉0．05）。结论猪同种异体肝肠联合移植中肝移植物可以诱导同源小肠移植物免疫耐受。Objective To study the effects of liver graft on the immune tolerance of intestinal allograft in auxiliary en-bloe liver-small bowel transplantation in pigs. Methods Seventy outbreed Landrace pigs were divided into 4 groups. Ten auxiliary liver-small bowel allotransplantations were performed in group A, B, C, respectively, and 5 segmental small bowel allotransplantations were performed in group D. Pigs were administered with routine and lower dose of eyclosporine and methylprednisolone in group B and C, respectively. No immunosuppressive agent was administered to pigs in group A and D. Results The initial time of acute rejection was obviously prolonged in group A than group D, and the acute rejection was milder in group A than group D （ P 〈 0.05 ）. There was no significant difference upon postoperative survival time, initial time of acute rejection and degree of acute rejection between group B and C （ P 〉 0.05 ）. Conclusions The immune tolerance of intestinal allograft can be induced by liver graft in auxiliary en-bloe liver-small bowel transplantation.

关 键 词：肝移植 肠移植 免疫耐受 猪 

分 类 号：R617[医药卫生—外科学] R392.4[医药卫生—临床医学]