创伤弧菌脓毒症大鼠肺组织脂多糖受体CD14mRNA、核因子-κB的基因动态表达  

Gene expression and the dynamic change of pulmonary lipopolysaccharide receptor CD14 and NF-κB in rats with Vibrio vulnificus sepsis

作　　者：郑阿迈[1] 陈玉熹[2] 李萌芳[1] 梁欢[1] 卢中秋[1] 

机构地区：[1]温州医学院第一附属医院急诊科,浙江温州325000 [2]温州市第二人民医院急诊科,浙江温州325000

出　　处：《温州医学院学报》2008年第6期534-536,540,共4页Journal of Wenzhou Medical College

基　　金：浙江省中医药管理局科研基金资助项目(2007CA085)

摘　　要：目的:探讨创伤弧菌(Vibrio vulnificus,VV)脓毒症大鼠肺组织脂多糖受体CD14 mRNA、核因子-κB(NF-κB)基因的表达及其动态变化。方法:SD雄性大鼠60只,随机分为6组,每组10只。第1组:正常对照组(NC组);第2～6组为VV组:予大鼠下肢皮下注射VV,造成VV脓毒症模型,以注射VV后2、6、9、12、16h各时间点分别为2～6组。取各组大鼠部分肺组织,用RT-PCR法测定大鼠肺组织不同时间点CD14 mRNA、NF-κB mRNA的表达。结果:NC组大鼠肺组织有少量CD14、NF-κB mRNA表达,分别为0.392±0.056和0.366±0.179。感染VV后大鼠肺组织CD14 mRNA表达增加,2、6、9、12、16h的表达量分别为0.976±0.180,1.148±0.218,1.026±0.033,0.988±0.129,0.970±0.125。大鼠肺组织NF-κBmRNA表达量也明显增加,2、6、9、12、16h的表达量分别为0.763±0.215,1.100±0.101,1.068±0.174,1.057±0.076和0.800±0.205。与NC组相比,二者均明显升高(P<0.05)。结论:VV脓毒症大鼠肺组织CD14、NF-κB的基因表达水平在脓毒症中明显升高,其与脓毒症的发生、发展密切相关,动态监测其改变对VV脓毒症发病过程具有一定的预警意义。Objective： To detect the Gene expression and the dynamic change of pulmonary li-popolysaccharide receptor CD14 and NF-KB in rats with Vibrio vulnificus （VV） sepsis. Methods： Sixty male Sprague-Dawley （SD） rats were equally divided into six groups randomly. The first group was named control group. W groups include the second to sixth group which were injected W to make W sepsis model. Samples were taken from control group and the second group to the sixth group at the timepoint of 2, 6, 9, 12, 16 h after injection respectively, the genes of pulmonary lipopelysaccharide receptor CD14 and NF-κB in rats lung were measured in the different time by the method of RT-PCR. Results： The Gene expression of pulmonary lipopolysaccharide receptor CDI4 and NF-κB in the control group was a low level （0.392±0.056, 0.366±0.179）, but they increased significantly at 2 h （0.976±0.180, 0.？63±0.215）, 6 h （1.148±0.218, 1.100±0.101）, 9 h （1.026±0.033, 1.068±0.174）, 12 h （0.988±0.129, 1.057±0.076）, 16 h （0.970±0.125, 0.800±0.205） after VV being injected （P〈0.05）. Conclusion： Our data suggest that the Gene expression of pulmonary lipopelysaccharide receptor CD14 and NF-κB elevation are associated with the development of VV sepsis. Monitoring the change of the Gene expression of pulmonary lipepolysaccharide receptor CD14 and NF-κB in the lung have the benefit of mastering the VV sepsis progress.

关 键 词：创作弧菌 脓毒症 脂多糖受体CD14 核因子-ΚB 基因 

分 类 号：R515.3[医药卫生—内科学]