EDS-40玻璃化液冻存小鼠囊胚的实验研究  

Study on the Cryopreservation of Mouse Blastocysts by the EDS-40 Vitrification

作　　者：鲁栋梁[1] 崔曙[1] 陈在贤[2] 

机构地区：[1]川北医学院附属医院泌尿外科,四川南充637000 [2]重庆医科大学附属第一医院泌尿外科,重庆400016

出　　处：《川北医学院学报》2008年第6期564-567,共4页Journal of North Sichuan Medical College

基　　金：重庆市卫生局科研课题资助项目(编号01-2-032)

摘　　要：目的研究玻璃化液EDS-40对冻贮小鼠囊胚的效果。方法(1)EFS-40和EDS-40玻璃化溶液的玻璃化测试。(2)随机将小鼠囊胚,在25°C室温下分别加入EDS-40和EFS-40玻璃化溶液,玻璃化后装管并迅速投入液氮中。对照组置于程序冷冻仪中进行程序化冷冻。(3)各组均冻存3个月,在25°C的水浴中复温后,用培养液反复洗涤后移入培养孔板培养,观察胚胎存活率及卵裂率。结果两种玻璃化溶液能达到玻璃化效果;EFS-40、EDS-40及对照组冷冻复温后的存活率分别为:82.14%、86.78%、69.6%;EFS-40、EDS-40及对照组冻胚的卵裂率分别为:66.96%、80.99%、54.4%。结论EDS-40较EFS-40玻璃化溶液冻存小鼠囊胚效果更好。Objective To study the eryoprotective effect of EDS-40 solution on mouse blastocysts. Methods We first tested the verif- ying effect of EDS - 40 and EFS - 40 solutions, then randomly chose blastocysts of female mice to vitrify in EFS - 40 or EDS - 40 solutions at 25℃ and immersed into liquid nitrogen. Embryos in control group were placed in program freezing equipment. All samples were eryopreserved for 3 months. The blastocysts were re-warmed rapidly in 25℃ water, and agitated gently, then blastoeysts were transferred into cultural medium and cultured. Then the survival rate and the merogenie rate were observed. Results Both EDS -40 and EFS -40 solutions had vitrifying effects. The survival rates of EFS -40, EDS -40 and control group were 69.6% , 82.14% and 86.78% respectively ; the merogenic rates of EFS - 40, EDS - 40 and control groups were 54.4% ,66.96% and 80.99% respectively. Conclusion The effect of EDS -40 solution is better than that of EFS -40..

关 键 词：玻璃化技术 玻璃化溶液 胚胎冻贮 

分 类 号：R699[医药卫生—泌尿科学]