胶原包裹挂线法家兔动脉血栓形成模型的建立与评价  

Establishment and evaluation of a rabbit model of arterial thrombosis by collagen encapsulated thread-drawing

作　　者：王东生[1] 陈方平[2] 贺石林[3] 黄细莲[2] 付斌[2] 肖长江[2] 唐发清[4] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,中药筛选工程技术中心,材料科学与工程博士后站,长沙410008 [2]中南大学湘雅医院血液科,长沙410008 [3]中南大学湘雅医学院血栓与止血研究室,长沙410078 [4]中南大学湘雅医院检验科,长沙410008

出　　处：《中南大学学报（医学版）》2008年第11期993-998,共6页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(30572455;30670990);中国博士后基金(20060390891);教育部新世纪优秀人才支持计划(NCET-06-0685);湖南省科技厅项目(2006FJ4243)~~

摘　　要：目的:建立胶原包裹挂线法家兔动脉血栓形成模型,并进行综合评价。方法:53只家兔随机分为正常组、挂线组、胶原包裹挂线组、胶原包裹挂线加阿司匹林处理组(阿司匹林组)、胶原包裹挂线加氯吡格雷处理组(氯吡格雷组)、胶原包裹挂线加阿司匹林和氯吡格雷处理(阿司匹林和氯吡格雷组)6组,分别进行相应的处理,观察在不同情况下组织病理学改变。用ELISA法(enzym link immunology assay,ELISA)检测1,6-二磷酸果糖二钠盐(D-fructose-1,6-diphosphate trisodium salt octahydrate,FDP)、D-二聚体(D-dimer)和组织因子(tissue factor,TF)。结果:组织病理学检查发现胶原包裹挂线组较挂线组血栓形成明显,血管内弹力膜损伤严重,用阿司匹林及氯吡格雷干预后,动脉血栓有大部分软化、溶解、吸收;胶原包裹挂线组与正常组、挂线组相比较,血栓湿重、干重、D-dimer、FDP及TF明显增高(P<0.01);3组药物干预后血栓湿重、干重及D-dimer、FDP和TF血浆水平均显著降低(P<0.01);与阿司匹林组、氯吡格雷组相比较,阿司匹林加氯吡格雷组能显著降低血栓湿重、干重、D-dimer和FDP水平(P<0.01);与阿司匹林组比较,阿司匹林加氯吡格雷组TF水平明显降低(P<0.05)。结论:胶原包裹挂线法动脉血栓模型明显优于单挂线法,且直观可靠、重复性好、成功率高,可反映抗栓药的药效,是一个理想的研究动脉血栓形成的动物模型。Objective To establish and evaluate a rabbit model of arterial thrombosis by modified thread-drawing. Methods Fifty-three rabbits were randomly divided into 6 groups： a normal group, a ligating group, a collagen encapsulated thread-drawing group, a aspirin group, a clopidogrel group, and an aspirin clopidogral group. The endovascular pathological changes in the rabbits were observed, and D-fructose-1 , 6-diphosphate trisodium salt octahydrate （ FDP ） , D-Dimer and tissue factor （TF）were detected with enzyme linked immunosorbent assay. Results In the thread-drawing group, thrombus was obvious, and the endovascular elastic membrane was injured seriously compared with the ligating group. After being treated with aspirin and clopidogrel, most arterial thrombus was softened, dissolved and absorbed. Compared with that in the modified thread-drawing group, wet and dry weight of thrombus increased, and the level of D-Dimer, FDP and TF also increased in the modified thread-drawing group （ P 〈 O. 01 ）. After being treated by aspirin and / or clopidogrel, the wet and dry weight of thrombus and the level of D-Dimer, FDP and TF decreased compared with the control （P 〈 0. 01 ）. Aspirin plus clopidogral could obviously reduce the wet and dry weight of thrombus, and reduce the level of D-Dimer and FDP （ P 〈 0.01 ）. Aspirin plus clopidogral could obviously inhibit the formation of TF compared with aspirin （ P 〈 0. 05 ）. Conclusion Arterial thrombosis model by collagen encapsulated thread-drawing which is visible, repeatable and effective is better than thread-drawing. It is suitable for screening anti-thrombosis drugs and evaluating their effect.

关 键 词：血栓形成模型 1 6-二磷酸果糖二钠盐 D-二聚体 组织因子 

分 类 号：R543[医药卫生—心血管疾病]