平肝潜阳药物对高血压病肝阳上亢证患者淋巴细胞蛋白质表达的影响  

Effect of calming the liver and suppressing the hyperactive YANG drugs on lymphocyte protein in hypertension patients with hyperactivity of liver-YANG

作　　者：钟广伟[1] 李炜[1] 罗艳红[2] 陈国林[1] 易振佳[1] 陈泽奇[1] 张臣[3] 彭川曼 

机构地区：[1]中南大学湘雅医院中西医结合研究所,长沙410008 [2]中南大学湘雅医院急诊科,长沙410008 [3]湖南省第二人民医院心血管内科,长沙410007 [4]湖南省长沙市第七人民医院心血管内科,长沙410006

出　　处：《中南大学学报（医学版）》2008年第11期1005-1011,共7页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(30500644;304072115)~~

摘　　要：目的:观察平肝潜阳药物对高血压病肝阳上亢证患者的临床疗效和血淋巴细胞蛋白质表达的影响,为识别鉴定平肝潜阳药物治疗高血压病肝阳上亢证相关蛋白质奠定基础。方法:26例高血压病肝阳上亢证患者采用平肝潜阳药物中药复方治疗。2个疗程结束后观察患者治疗前后的中医症状记分、血压,利用固相pH梯度双向凝胶电泳分离正常人及高血压病肝阳上亢证患者治疗前后淋巴细胞总蛋白质,用ImageMaster 2DE软件分析双向电泳图谱,以识别差异表达的蛋白质。结果:平肝潜阳药物中药复方治疗总有效率为88.5%;改善高血压病肝阳上亢证患者症状,如头痛、眩晕、口干和烦躁易怒等;平肝潜阳药物中药复方有降低血压的作用,治疗前后比较差异有统计学意义(P<0.05)。正常人、高血压病肝阳上亢证患者治疗前、治疗后3种淋巴细胞凝胶蛋白质点数依次为527±41,559±62和543±59。高血压病肝阳上亢证患者与正常人比较,有15个蛋白质表达下调,10个蛋白质表达上调;高血压病肝阳上亢证治疗前后比较,表达下调15个蛋白质中有12个表达增强,而表达上调10个蛋白质中有6个表达降低。结论:平肝潜阳药物中药复方有缓和的降压作用,有较好的改善中医症状的作用;平肝潜阳药物治疗高血压病肝阳上亢证可能是通过调节血淋巴细胞蛋白质的表达而实现的。Objective To explore the effect of calming the liver and suppressing the hyperactive YANG drugs on the lymphocyte protein and clinical efficacy in the hypertension patients with hyperactivity of liver-YANG, and to identify the therapy. Methods Twenty-six hypertension patients with hyperactivity of liver-YANG were treated by calming the liver and suppressing the hyperactive YANG drugs for 2 courses. Symptoms of Chinese medicine and blood pressure were observed, and the separated lymphocyte total protein of normal and hypertensions before and after the treatment were examined by the solid-state pH gradient 2-dimensional gel electrophoresis. The differences of the protein expression were analyzed by ImageMaster 2DE analysis software with two-way patterns. Results The total efficiency rate of calming the liver and suppressing the hyperactive YANG drugs was 88.5 % , and the drugs could significantly relieve the symptoms, such as headache, dizziness, dry mouth, irritability, etc. Calming the liver and suppressing the hyperactive YANG drugs could also remarkably reduce the blood pressure, with significant different between pre-treatment and post-treatment （ P 〈 0.05 ）. The average spots of lymphocyte gel proteins in the normal and the hypertension patients with syndrome of hyperactivity of liver-YANG before and after the treatment were 527 ±41 , 559±62, and 543±59, respectively. Compared with normal people, the expression of 15 proteins was down-regulated, and 10 upregula of liver-YANG. Compared with the pre ted in the hypertension patients with syndrome of hyperactivity -treatment, the expression of 12 proteins was increased in the 15 down-regulated proteins, and 6 decreased in the 10 up-regulated proteins after the treatment in the hypertension patients with syndrome of the hyperactivity of liver-YANG. Conclusion Calming the liver and suppressing the hyperactive yang drugs may mildly depress the blood pressure and improve the symptoms of Chinese medicine. The effect of drugs in treating hypertension may prob

关 键 词：平肝潜阳药物 肝阳上亢证 2-DE图谱 高血压病 淋巴细胞 

分 类 号：R259[医药卫生—中西医结合]