PRL-3及其同源异形体重组杆粒的构建和鉴定  

Construction of PRL-3 gene recombinant baculovirus vector

作　　者：何太平[1] 熊亮[1] 莫丽儿[2] 梁念慈[1,2] 

机构地区：[1]广东医学院生物化学与分子生物学研究所 [2]广东天然药物研究与开发重点实验室,广东湛江524023

出　　处：《广东医学院学报》2008年第5期489-491,495,共4页Journal of Guangdong Medical College

基　　金：广东省科技计划项目(No.2007B030702003);广东省中医药局课题(No.1060044)

摘　　要：目的构建PRL-3及其同源异形体重组杆状病毒表达载体,为下一步表达PRL-3及其同源异形体蛋白并从中草药活性成分中筛选出PRL-3抑制剂奠定基础。方法应用RT-PCR方法从高转移卵巢癌细胞HO-8910PM扩增PRL-3及其同源异形体编码序列,克隆到供体质粒pFastBac HTB,在DH10Bac菌中进行同源重组,经3种抗生素和蓝白斑筛选,得到Bacmid-HTB-PRL-3和Bacmid-HTB-PRL-3-variant,PCR鉴定其正确性。结果与结论成功构建了PRL-3及其同源异形体重组杆粒。Objective To construct recombinant Bacmid of PRL-3 and its variant. Methods PRL-3 and its variant cDNA were amplified from total RNA of HO-8910PM by RT-PCR, and inserted into plasmid pFastBacHTB. The recombinant Bacmid of PRL-3 and its variant was screened by three antibiotics and Bluo-gal, confirmed by PCR. Results and Conclusion The re- combinant Bacmid of PRL-3 and its variant had been successfully constructed. It provides a basis for further study of expression of PRL-3 and its variant proteins and screening small molecule inhibitors from active components of Chinese traditional and herbal drugs.

关 键 词：PRL-3 杆状病毒 基因重组 

分 类 号：Q784[生物学—分子生物学]