常规剂量地塞米松对大鼠脑损伤后脑组织核转录因子-κB表达的影响  

Effect of routine dose of dexamethasone on the expression of the transcription factor NF-κB in rat brain tissue after traumatic brain injury

作　　者：尹延庆[1] 陈兵[1] 吴华伟[1] 彭浩[1] 龙霄翱[1] 

机构地区：[1]广东医学院附属医院神经外科,广东湛江524001

出　　处：《广东医学院学报》2008年第5期508-509,512,共3页Journal of Guangdong Medical College

基　　金：2008年广东省医学科研基金立项课题(No.A2008484);2008年湛江市科技攻关项目(No.湛科2008C05006)

摘　　要：目的了解常规剂量地塞米松对大鼠脑损伤后脑组织核转录因子-κB(NF-κB)表达的影响。方法将60只SD大鼠随机分为空白组、假手术组、地塞米松组3组,每组20只。局灶性脑损伤后,空白组和假手术组分别腹腔注射0.1mL/(kg.d)生理盐水,地塞米松组腹腔注射地塞米松0.1mg/(kg.d)。各组动物分别在6、24、48和96h处死5只取脑制片,NF-κB表达用免疫组化检测。结果空白组各时间点NF-κBp65蛋白表达均高于假手术组(P<0.05或0.01);地塞米松组6、48h均低于空白组(P<0.01),两组24、96h时点差异均无统计学意义(P>0.05)。结论脑损伤后受损脑组织NF-κB表达升高,常规剂量地塞米松可以抑制NF-κB表达而有效保护创伤脑组织。Objective To observe the effect of routine dose of dexamethasone on the expression of the transcription factor NF-κB in rat brain tissue after traumatic brain injury. Methods 60 SD rats were randomly divided into three groups, each with 20 rats. In the blank control group, the injured animals received intraperitoneal injection of 0. 1mL/（kg·d） of normal saline; In sham-operated group, the sham-operated animals received intraperitoneal injection of 0. 1mL/（kg·d） of normal saline; In dexamethasone group, the injured animals received intraperitoneal injection of 0. 1mg/（kg·d） of dexamethasone. Five brain tissue samples were obtained at 6, 24, 48 and 96h after trauma in each group. Immunohistochemistry was used to detect the expression of NF-κB in the traumatic brain tissue. Results The expression levels of NF-κBp65 protein in blank control group at all time points were higher than those in shanl-operated group（P〈0.05 or 0.01）. The expression levels of NF-κBp65 protein in dexamethasone group at 6 and 48h were significantly lower than those in blank control group （ P 〈 0.01 ）, but not at 24 and 96h time point（P 〉0.05）. Conclusion The expression of NF-κB was increased in the traumatic brain tissue, and the routine dose dexamethasone could effectively protect traumatic brain tissue by inhibiting the expression of NF-κB.

关 键 词：脑损伤 核转录因子-ΚB 地塞米松 

分 类 号：R651.15[医药卫生—外科学]