全身照射对小鼠骨髓间充质干细胞细胞衰老相关指标的影响  

Effect of Total Body Irradiation on Cellular Senescence Related Indexes of Bone Marrow Mesenchymal Stem Cells

作　　者：马杰[1] 王宏兰[2] 李静[2] 史明霞[2,3] 李炳宗[2,4] 陈斌[2] 胡建立[2] 赵春华[2] 孙慧[1] 

机构地区：[1]郑州大学第一附属医院血液科,河南郑州450052 [2]中国医学科学院北京协和医学院基础医学研究所组织工程研究中心,北京100005 [3]昆明医学院第一附属医院血液科 [4]苏州大学附属第二医院血液科

出　　处：《中国实验血液学杂志》2008年第6期1387-1391,共5页Journal of Experimental Hematology

基　　金：国家高技术研究发展计划863资助项目(No.2002AA205061);国家杰出青年基金资助项目(No.30125018)

摘　　要：为了探讨小鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMMSCs)在放射损伤后细胞衰老(cellular senescence)的细胞和分子水平相关指标的变化,本研究采用全身照射(total body irradiation,TBI)小鼠模型,观察4 Gy TBI后4周内不同时间点小鼠BMMSCs的形态学和衰老相关β-半乳糖苷酶(senescence-associatedβ-galactosidase,SA-β-gal)的变化,用流式细胞术分析TBI对BMMSCs细胞周期分布的影响,用实时定量RT-PCR检测细胞衰老相关基因p16INK4a、p21Cip1/Waf1、p53和TGF-β1在TBI前后的表达变化。结果显示,4 Gy TBI后4周内小鼠BMMSC的形态和SA-β-gal的表达无明显变化,也未出现细胞衰老相关的细胞周期阻滞和衰老相关基因表达增高。结论:小鼠BMMSCs在4 Gy TBI后近期内未出现细胞衰老相关的分子水平的改变。The aim of this study was to explore the changes in cellular senescence related indexes of bone marrow mesenchymal stem cells （BMMSCs） after total body irradiation （TBI）. At different time points after 4 Gy irradiation, BMMSCs were isolated from male C57BL/6 mice and cultured. Morphylogy, senescence-associated β-galaetosidase （SA-β-gal） staining and cell cycle analysis were used to evaluate the changes in BMMSCs at cellular level while realtime RT-PCR was used to detect the alterations in senescence related gene expression including p16^INKα ,p21^Cipl/Wafl ,p53 and TGF-β1. The results showed that within 4 weeks after exposure to 4 Gy TBI, the morphology of BMMSCs and the expression level of SA-β-gal were not significantly changed, the cellular senescence-ralated cell cycle arrest was not occured and the senescence related gene expression level was not increased. It is concluded that at the early stage after 4 Gy TBI, the related molecular level of cellular senescence in BMMSCs is not Changed.

关 键 词：全身照射 骨髓间充质干细胞 细胞衰老 

分 类 号：Q691[生物学—生物物理学]