海藻糖抑制高浓度葡萄糖诱导的红细胞磷脂酰丝氨酸暴露、渗透脆性增高和膜脂质过氧化损伤的研究  

Inhibitory Effect of Trehalose on Phosphatidylserine Exposure,Osmotic Fragility and Membrane Lipid Peroxidation Damage of Erythrocytes Induced by High Concentration of Glucose

作　　者：权国波[1] 韩颖[1] 杨超[1] 扈文博[1] 刘敏霞 刘安[1] 王艳[1] 王捷熙[1] 

机构地区：[1]军事医学科学院野战输血研究所,北京100850

出　　处：《中国实验血液学杂志》2008年第6期1442-1446,共5页Journal of Experimental Hematology

基　　金：国家自然科学基金项目;编号30400518;北京市自然科学基金;编号7072059;军队"十一五"青年学者项目;编号06Q90

摘　　要：尽管高浓度葡萄糖孵育有利于深低温或冰冻干燥保存人红细胞的存活,但是高浓度葡萄糖仍然对红细胞造成一定损伤。本研究探讨海藻糖对高浓度葡萄糖导致的红细胞损伤的影响。将红细胞于含有一定浓度海藻糖的葡萄糖缓冲液中孵育3小时后,用流式细胞术分析红细胞磷脂酰丝氨酸(PS)分布和细胞渗透脆性,用TBA法检测膜脂质过氧化损伤。结果表明:葡萄糖可以导致红细胞PS暴露、脂质过氧化损伤和细胞渗透脆性增高,而且其效率和葡萄糖浓度和温度密切相关。然而,海藻糖却可以很好地抑制高浓度葡萄糖诱导的红细胞PS暴露、渗透脆性增高和过氧化损伤。随着海藻糖浓度的增加,红细胞PS标记率、丙二醛(MDA)浓度和细胞碎片率呈逐步下降的趋势。结论:高浓度葡萄糖可以导致细胞PS暴露、脂质过氧化损伤和渗透脆性增高,而且这3种损伤之间存在一定关系;海藻糖可以有效地抑制高浓度葡萄糖导致的红细胞PS暴露、渗透脆性增高以及过氧化损伤,这对于应用小分子糖类保存红细胞研究具有重要意义。Though high concentration of glucose can benefit the survival of lyophilized human red blood cells, the high concentration of glucose can result in serious damage of red blood cells. This study was aimed to investigate the inhibitory effect of trehalose on damage of red blood cells induced by high concentration of glucose. After incubation with the high concentration of glucose buffer containing different concentrations of trehalose for three hours at 37℃, the phosphatidylserine exposure and the osmotic fragility of cells were analyzed by flow cytometry and the lipid peroxidation of membrane was evaluated by TBA method. The results showed that the high concentration of glucose could lead to phosphatidylserine exposure, osmotic fragility increase, and lipid peroxidation damage which were dependent on the glucose concentrations and incubation temperature. However, trehalose could effectively prevent the phosphatidylserine exposure, osmotic fragility increase, and lipid peroxidation gamage induced by high concentration glucose. With increase of the trehalose concentrations. As the trehalose concentration increases, the phosphatidylserine exposure, malondialdehyde concentration and cell debris rate decreased gradually. In conclusion, the high concentration of glucose can lead to phosphatidylserine exposure, osmotic fragility increase, and lipid peroxidation damage of red blood cells. However, trehalose can inhibit the damaging effects of high concentration of glucose on red blood cells, which may be useful for the application of sugars to lyophilization of red blood cells.

关 键 词：海藻糖 葡萄糖 红细胞 磷脂酰丝氨酸暴露 渗透脆性 膜脂质过氧化损伤 

分 类 号：R331.141[医药卫生—人体生理学]