NGF增强小鼠实验性免疫功能低下研究  

Enhanced Effect of Nerve Growth Factor Injection Solution on Experimental Immunosuppression in Mice

作　　者：祁岩超[1] 刘振寰[2] 柴铁劬[3] 

机构地区：[1]广州医学院附属肿瘤医院,510095 [2]广州中医药大学附属南海妇产儿童医院 [3]广州中医药大学针灸推拿学院

出　　处：《医学研究杂志》2008年第12期33-36,F0003,共5页Journal of Medical Research

基　　金：国家自然科学基金项目(30740074);广东省自然科学基金项目(5002028);广州市卫生局中西医结合课题(2005A040)

摘　　要：目的研究神经生长因子(NGF)注射液对小鼠实验性免疫功能低下的影响。方法以环磷酰胺(Cy80mg/kg,皮下注射)造成小鼠免疫功能低下的动物模型,采用碳粒廓清法、单向免疫扩散法、二硝基氟苯诱导迟发型超敏反应法、3H-TdR参入法以及间接免疫光法测定有关免疫指标。结果NGF注射液[0.01、0.1mg/(kg.d)×10d,im]可使小鼠降低的K值、a值、血清IgG、耳片肿胀度、ConA诱导的T淋巴细胞增生反应值等明显升高;同时可使小鼠外周血减少的L3T4+细胞增多,从而使降低的L3T4+/Lyt-2+细胞比值增高。结论NGF注射液对小鼠实验性免疫功能低下有明显的增强作用,从而为放、化疗所致神经损伤肿瘤病人的临床应用提供了理论依据。Objective To investigate the effect of nerve growth factor （NGF） injection solution on immunosuppression in mice. Methods The immunosuppressed mice were induced by cyclophosphamide （Cy, 80mg/kg,sc）. The immunomodulatory effects of the drug were measured by cleaning carbon particle method, simple diffusion method, delayed type hypersensitivity （DTH） , 3^H -TdR entering method and indirect immunofluorescence assay. Results The k value; a value, the level of serum IgG and ear swelling were remarkably decreased by NGF injection [0.01, 0. 1 mg/（kg · d） × 10d, im]. In addition, the number of L3T4^＋ Cells of peripheral blood and the ratio of L3T4^＋/Ly -2^＋ cell increase. Conclusion NGF has an enhanced effect on experimental immunosuppression in mice,which provides a theoretical basis for NGF treating tumor patients with nervous injure by chemotherapy or radiotherapy.

关 键 词：NGF 环磷酰胺 免疫调节 T淋巴细胞亚群 小鼠 

分 类 号：R285.5[医药卫生—中药学] R651.3[医药卫生—中医学]