钛颗粒刺激巨噬细胞而释放的肿瘤坏死因子对人工关节松动的作用  

The effect of TNF-α release in macrophages stimulated with titanium particles on the aseptic loosening of artificial joints

作　　者：金山[1] 

机构地区：[1]吉林省延边第二人民医院骨科,133001

出　　处：《中国实用医药》2008年第34期22-24,共3页China Practical Medicine

摘　　要：目的分析钛颗粒刺激巨噬细胞而释放的肿瘤坏死因子在人工髋关节术后引起骨溶解而假体松动的分子生物学机制。方法采用钛颗粒刺激巨噬细胞而释放TNF-α模型及酶联免疫吸附剂测定、蛋白质印迹、凝胶迁移率实验方法来研究其生物分子学机制。结果鼠类巨噬细胞受钛颗粒刺激而释放TNF-α,而JNK抑制剂SP600125和NF-κB抑制剂MG132明显抑制了钛颗粒诱导的TNF-α释放,但p38抑制剂无此作用,甚至,钛颗粒诱导巨噬细胞而激活AP-1和NF-κB。结论钛颗粒诱导的TNF-α释放与JNK/AP-1及NF-κB通路有关。Objective To analyse the mechanism of osteolysis after total hip replacement by tumor necrosis factor-α（TNF-α） release in macrophages stimulated with particles. Methods A model of TNF-α release by stimulating macrophages with titanium particles was made and study the mechanism of TNF-α release by ELISA/West blot/EMSA. Results TNF-α was released in murine macrophage stimulaged by titanium（Ti）particles and specific inhibitors of JNK（SP600125） and NF-κB （MG132） ,but not p38 （SB203580） dramatically inhibited Ti-stimulated TNF-α release. Moreover, transcriptional activation of AP-1 and NF-κB was also induced by Ti particles. Conclusion These results demonstrate that Ti particle-induced TNF-α release is mediated through JNK/AP-1 as well as NF-κB pathways.

关 键 词：人工髋关节置换术 钛颗粒 骨溶解 肿瘤坏死因子 

分 类 号：R687.4[医药卫生—骨科学]