Vitros950生化检测系统最大稀释度测定  

Determination of the maximum dilution in Vitros 950 biochemistry system

作　　者：姚少羽[1] 孙艳虹[1] 高玲[1] 喻雄文[1] 温俊杰[1] 

机构地区：[1]中山大学附属第一医院检验医学部,广州510080

出　　处：《中国实用医药》2008年第34期31-34,共4页China Practical Medicine

摘　　要：目的测定Vitros950生化检测系统的最大稀释度。方法对肌酐(CREA)、总胆红素(TBIL)、肌酸激酶(CK)、淀粉酶(AMYL)、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、总蛋白(TP)、白蛋白(ALB)这8个项目的高值标本分别用生理盐水和低值患者血清进行不同程度的稀释,检测并计算预期值与实测值之间的误差,以美国CLIA’88能力验证计划的分析质量要求为标准判定结果是否可接受,以确定这8个项目分别用生理盐水和低值患者血清可以稀释的最大程度。结果用低值患者血清稀释8个项目的最大稀释度均达到了1∶128;用生理盐水稀释,CREA的最大稀释度为1∶128,TBIL为1∶4,CK为1∶8,AMYL为1∶32,AST为1∶64,ALT为1∶16,TP为1∶4,ALB为10∶13。结论生理盐水稀释患者血清作为临床检测较为方便,但是需要注意最大稀释倍数的限制。低值患者血清具有更理想的基质,能达到更大的稀释倍数。Objective To investigate the maximum dilution of biochemistry analysis system of Vitros 950. Methods The samples were diluted separately with 0.9% NaCl or serum at low value of the items and then the analytes in the diluted samples were tested. The standard error were compared between two different dilutors and then the most suitable dilution rate were determined according to the standard of CLIA＇ 88. Results The most suitable dilution of Crea, TBil, CK, Amy, AST, ALT, TP and ALB using serum at low value as dilutor reaches 1 ： 128,while the most suitable dilution using 0. 9% NaCl much lower. Conclusion Low value serum is more suitable as dilutor than 0. 9% NaCl in Vitros 950 biochemistry system.

关 键 词：最大稀释度 临床可报告范围 基质效应 

分 类 号：R446.1[医药卫生—诊断学]