Inactivation of bovine immunodeficiency virus by photodynamic therapy with HMME  

作　　者：阴慧娟 李迎新 邹朝晖 乔文涛 姚雪 苏旸 郭红彦 

机构地区：[1]Laser Medicine Laboratory Biomedical Engineering Department of Tianjin Medical University [2]College of Life Sciences Nankai University

出　　处：《Chinese Optics Letters》2008年第12期944-946,共3页中国光学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.60678047);the Science Foundation of Tianjin(No.05YFJZJC02300)

摘　　要：To investigate the effect of photodynamic therapy （PDT） with hematoporphrin monomethyl ether （HMME） on bovine immunodeficiency virus （BIV） can provide the basis theory for photoinactivation of human immunodeficiency virus （HIV）. To assess the protection of HMME-PDT on the cell line Cf2Th infected with BIVR29 by 3-（4,5）-dimethylthiahiazol-2-yl-3,5-di-phenytetrazolium bromide （MTT） with power density of 5 and 25 mW/cm^2 and energy density from 0.6 to 3 J/cm^2. To observe the inhibition of membrane fusion using a new reporter cell line BIVE by fluorescence microscope. HMME-PDT has significant protectant effects on Cf2Th-BIVR29 with both power densities, especially in the group of high power density. Fluorescent microscope shows that there is no significant difference between the group of PDT and control, which means PDT could not inhibit the BIV-mediated membrane fusion.To investigate the effect of photodynamic therapy （PDT） with hematoporphrin monomethyl ether （HMME） on bovine immunodeficiency virus （BIV） can provide the basis theory for photoinactivation of human immunodeficiency virus （HIV）. To assess the protection of HMME-PDT on the cell line Cf2Th infected with BIVR29 by 3-（4,5）-dimethylthiahiazol-2-yl-3,5-di-phenytetrazolium bromide （MTT） with power density of 5 and 25 mW/cm^2 and energy density from 0.6 to 3 J/cm^2. To observe the inhibition of membrane fusion using a new reporter cell line BIVE by fluorescence microscope. HMME-PDT has significant protectant effects on Cf2Th-BIVR29 with both power densities, especially in the group of high power density. Fluorescent microscope shows that there is no significant difference between the group of PDT and control, which means PDT could not inhibit the BIV-mediated membrane fusion.

关 键 词：HMME BIV AIDS 

分 类 号：S852.65[农业科学—基础兽医学]