Investigation of the topological shape of bovine serum albumin in solution by small-angle x-ray scattering at Beijing synchrotron radiation facility  

作　　者：董淑强 李丽琴 刘鹏 董宇辉 陈熙萌 

机构地区：[1]Nuclear Science and Technology Department of Lanzhou University [2]Institute of High Energy Physics, Chinese Academy of Sciences

出　　处：《Chinese Physics B》2008年第12期4574-4579,共6页中国物理B（英文版）

基　　金：Project supported by the Chinese Academy of Science Innovation Key Project (Grant No KJCX2-SW-N06)

摘　　要：This paper reports that at a newly constructed small-angle x-ray scattering station of Beijing Synchrotron Radia- tion Facility, the topological shape of ligand-free bovine serum albumin in solution has been investigated. An appropriate scattering curve is obtained and the calculated value of the gyration radius is 31.2~=t=0.25 ~_ （11=0.1 nm） which is co- incident with other ones＇ results. It finds that the low-resolution structure models obtained by making use of ab initio reconstruction methods are fitting the crystal structure of human serum albumin very well. All of these results perform the potential of the beamline to apply to structural biology studies. The characteristics, the defects, and the improving measures of the station in future are also discussed.This paper reports that at a newly constructed small-angle x-ray scattering station of Beijing Synchrotron Radia- tion Facility, the topological shape of ligand-free bovine serum albumin in solution has been investigated. An appropriate scattering curve is obtained and the calculated value of the gyration radius is 31.2~=t=0.25 ~_ （11=0.1 nm） which is co- incident with other ones＇ results. It finds that the low-resolution structure models obtained by making use of ab initio reconstruction methods are fitting the crystal structure of human serum albumin very well. All of these results perform the potential of the beamline to apply to structural biology studies. The characteristics, the defects, and the improving measures of the station in future are also discussed.

关 键 词：small-angle x-ray scattering ab initio methods bovine serum albumin Beijing synchrotron radiation facility 

分 类 号：Q51[生物学—生物化学] O434.1[机械工程—光学工程]