骨髓间充质干细胞自体移植时间对兔急性肾功能衰竭治疗效果的影响  

The therapeutic effectiveness of autologous bone marrow mesenchymal stem cells transplantation for acute renal failure in rabbits

作　　者：李玲莉[1] 丁国华[1] 张茨[2] 魏捷[3] 

机构地区：[1]武汉大学人民医院泌尿系病研究所,430060 [2]武汉大学人民医院泌尿外科,430060 [3]武汉大学人民医院急诊科,430060

出　　处：《中华实验外科杂志》2008年第12期1574-1576,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30670985）

摘　　要：目的观察骨髓间充质干细胞（MSCs）自体移植对兔急性肾功能衰竭的治疗作用并探讨不同移植时间对其治疗效果的影响。方法骨髓穿刺抽取新西兰大耳白兔骨髓，分离、培养、扩增MSCs。30只兔通过夹闭双肾动脉90min后再灌注制作急性肾功能衰竭模型，随机分为移植A组、移植B组和未治疗C组，每组10只。移植A组在恢复血流即刻，移植B组在恢复血流后72h将BrdU标记的自体MSCs经颈静脉回输移植，未治疗C组仅制作急性肾功能衰竭模型。于造模后21d处死兔，比较各组的存活率、肾功能及肾组织形态学改变。结果与未治疗组比较，移植组肾功能较快恢复（P〈0．05），肾组织损伤明显改善（P〈0．05）。移植A组的效果优于移植B组（P〈0．05）。结论骨髓间充质干细胞自体移植能有效治疗缺血再灌注损伤引起的急性肾功能衰竭，早期移植的效果好。Objective To evaluate the therapeutic effects of autologous bone marrow mesenchyreal stem ceils （MSCs） transplantation for acute renal failure in rabbits and the impact of the different transplantation opportunity on therapeutic effects. Methods Bone marrow MSCs were isolated and cultured in vitro. Acute renal failure was induced by clamping bilateral renal artery for 90 min ,then the renal circulation restored. Thirty rabbits were divided randomly into three groups ： transplanted group A （ n = 10） ,transplanted group B（ n = 10） and untreated group C （n = 10）. In transplanted group A autologous MSCs labeled with BrdU were transplanted via carotid vein immediately after the resumption of circulation in the kidney, and in transplanted group B MSCs were transplanted 72 h after the resumption of circulation in the kidney. In untreated group C only the model of acute renal failure was made. Twenty-one days after the operation all rabbits were sacrificed. The survival rate among groups were analyzed, and renal function and renal structure were evaluated. Results Compared with untreated group C, MSCs transplanted groups have faster recovery of renal function and renal structure （ P 〈 0.05）. The therapeutic effects of transplanted group A were better than those of transplanted group B （ P 〈 0.05 ）. Conclusion Autologous bone marrow mesenchymal stem cells transplantation is effective for treatment of acute renal failure in rabbits. Bone marrow MSCs transplantation at early stage might have better therapeutic effects.

关 键 词：骨髓间充质干细胞 急性肾功能衰竭 再灌注损伤 自体移植 

分 类 号：R692.5[医药卫生—泌尿科学] R329.2[医药卫生—外科学]