气-液面培养对角质形成细胞分化的影响  

The influence of air-liquid interface culture on differentiation of keratinocytes

作　　者：马忠锋[1] 柴家科[1] 杨红明[1] 梁黎明[1] 许明火[1] 

机构地区：[1]解放军总医院第一附属医院烧伤整形科,全军烧伤研究所,北京100037

出　　处：《中华实验外科杂志》2008年第12期1577-1578,共2页Chinese Journal of Experimental Surgery

基　　金：全军医学科研“十一五”计划专项课题（06Z054）;首都医学发展科研基金（2005-2040）;北京市科技计划研发攻关课题（H060920050830）

摘　　要：目的研制角质形成细胞-胶原-猪去细胞真皮基质（PADM）组织工程皮肤，观察气．液面培养对角质形成细胞分化及细胞因子基因表达的影响。方法将角质形成细胞种植在真皮基质胶原面进行气-液面培养和浸没式培养。采用逆转录．聚合酶链反应（RT—PCR）技术，对比研究两种培养方法获得的细胞膜片bFGF基因表达差异。结果气-液面培养获得的角质形成细胞层结构与正常皮肤相似，细胞分化良好。单纯浸没式培养角质形成细胞层较薄，分化较差。气-液面培养获得的细胞膜片bFGF mRNA表达量明显高于单纯浸没式培养（t=3．825，P〈0．01）。结论气．液面培养可促进角质形成细胞的生长、分化及复层表皮结构形成，影响细胞因子的基因表达。Objective To prepare tissue-engineered skin containing keratinocytes and dermal matrix prepared by Collagen-PADM, and observe the influence of air-liquid interface culture on differentiation and eytokine gene expression of keratinocytes. Methods The keratinocytes were inoculated on the collagen surface of the dermal matrix by air-liquid interface culture and imerged culture. By using RT-PCR method, the bFGF gene expression in keratinoeytes sheets was compared. Results The structure of keratinocytes by air-liquid interface culture was similar to that of the normal skin,and keratinoeytes differentiated well. By using imerged culture method, the structure of keratinoeytes was thin, and keratinocyte differentiation was immature. The bFGF-RNA expression of keratinocytes sheet was higher by air-liquid interface culture （ t = 3. 825,P 〈 0.01 ）. Conclusion Air-liquid interface culture may facilitate the growth, differentiation and multiple stratum of keratinoeytes, and influence the eytokine gene expression.

关 键 词：角质形成细胞 组织工程 皮肤 基因表达 

分 类 号：R318.0[医药卫生—生物医学工程]