壳聚糖-胶原生物膜介导雪旺细胞联合神经干细胞桥接周围神经缺损  

Connecting the defects of sciatic nerve by transplantation of Schwann cells and neural stem cells embedded to the chitosan-collagen complex film

作　　者：冯世庆[1] 李崴[2] 孔晓红[3] 陈家童[3] 曹富江[1] 郭世绂[1] 

机构地区：[1]天津医科大学总医院骨科,300052 [2]天津市肿瘤医院 [3]南开大学

出　　处：《中华实验外科杂志》2008年第12期1630-1632,共3页Chinese Journal of Experimental Surgery

基　　金：教育部新世纪人才支持计划（NCET-06-0251）;天津市应基础和前沿技术支持计划（07JCYBJC10200）

摘　　要：目的观察以壳聚糖-胶原偶联的生物膜为载体，联合移植雪旺细胞（SCs）与神经干细胞（NSCs）在坐骨神经损伤修复过程中所起的作用，评价该方法的疗效。方法分离纯化SCs和NSCs，传代4代后共培养于壳聚糖-胶原生物膜上。32只Wistar成鼠（体重350—400g）建立坐骨神经缺损动物模型，随机分为4组：空白组；SCs移植组；NSCs移植组；NSCs联合SCs移植组。14周后观察Cy3荧光素染色、MBP免疫酶染色及苏木素-伊红（HE）染色的结果。结果BDA示踪显示术后联合移植组和SCs组有较多的神经纤维穿过缺损部位，大鼠右后肢感觉和运动功能恢复较其他组明显，修复神经段神经传导速度比值也大于其他组（P〈0．05）。SCs组和联合移植组间差异无统计学意义（P〉0．05）。结论NSCs和SCs与壳聚糖-胶原生物膜相容性良好，壳聚糖-胶原生物膜介导的SCs联合NSCs桥接周围神经缺损可使部分神经再生。Objective To evaluate the effect bridging＇the of nerve in Wistar rats of defects sciatic by transplantation of Schwann ceils （SCs） and neural stem cells （NSCs） embedded to the chitosan-collagen complex film. Methods SCs and NSCs were isolated and purified. These clones were passaged continuously for 4 generations. Then the two types of cells were embedded to the chitosan-collagen complex film. Thirty-two adult Wistar rats,weighing 350400 g, were divided into 4 groups at random：Blank group, SCs group,NSCs group and SCs ＋ NSCs group. An about 10 mm gap of right sciatic nerve was cut in all rats. The films planted by SCs and/or NSCs or none were transplanted to bridge the sciatic nerve defects. Fourteen weeks later MBP was detected by using cy3 fluorescence staining, HE staining, and immunohisto-chemistry staining. The velocity of electrical conduction of sciatic nerves was calculated. Results The recovery of sense and motion function in SCs ＋ NSCs group and SCs group were better than the other two groups. SCs ＋ NSCs group and SCs group were superior to the other two groups on neurofibril regeneration and velocity of electrical conduction of sciatic nerve （ P 〉 0.05）. But there was no significant difference between SCs group and SCs ＋ NSCs group. Conclusion The two types of cells have a good compatibility with the chitosan-collagen complex film. Transplantation of SCs and NSCs embedded to the chitosan-collagen complex film can guide the regeneration of neurofibrils.

关 键 词：雪旺细胞 神经干细胞 壳聚糖 胶原 

分 类 号：R651.2[医药卫生—外科学]