反义转化生长因子-β1质粒对肌腱术后粘连形成的影响  

Effects of antisense TGF-β1 recombinant plasmid on adhesion formation of flexor tendon

作　　者：夏长所[1] 李富江[1] 张宏[2] 陈玉华[1] 杨选影[2] 洪光祥[3] 

机构地区：[1]青岛大学医学院附属医院骨科,266003 [2]青岛大学医学院附属医院药剂科,266003 [3]华中科技大学同济医学院附属协和医院手外科

出　　处：《中华实验外科杂志》2008年第12期1637-1639,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察反义转化生长因子（TGF）-β1质粒对肌腱粘连的治疗作用。方法将24只成年新西兰大白兔随机分成3组，行中趾屈指肌腱切断吻合术，腱鞘内分别注入生理盐水、反义TGF-1脱氧寡核苷酸和反义TGF-β1质粒，4周后取出肌腱进行生物力学测试；48只成年新西兰大白兔随机分成2组，行中趾屈指肌腱切断吻合术，腱鞘内分别注入生理盐水或反义TGF-β1质粒，于术后7、14、28、56d取出肌腱，原位杂交方法测定肌腱TGF-β1和Ⅰ型胶原mRNA的表达。结果生物力学结果表明使用反义TGF-β1质粒能有效的改善屈指肌腱术后的粘连；原位杂交显示反义TGF-β1质粒能有效的降低每个时间点TGF-β1和Ⅰ型胶原的表达。结论反义TGF-β1质粒能有效的抑制TGF-β1在肌腱损伤修复中的作用，减少粘连的形成。Objective To investigate the preventive effect of antisense TGF-β1 recombinant plasmid on flexor tendon adhesion formation. Methods Twenty-four aduh New Zealand White rabbits were divided into three groups randomly after anastomonsis operation of flexor tendons：normal saline group,TGF- β1 oligonucleotide group, antisense TGF-β1 recombinant plasmid group. Tendons were harvested at 4th week and biomechanics testing was conducted. Fouty-eight adult New Zealand White rabbits were divided into two groups randomly after anastomonsis operation of flexor tendons： normal saline group, antisense TGF-β1 recombinant plasmid group. Tendons were harvested on the postoperative day 7,14,28 and 56 and the mRNA expression of TGF-β1 and Collagen Ⅰ was detected by in situ hybridization. Results The results of biomechanics testing indicated that antisense TGF-β1 recombinant plasmid could prevent adhesion formation of flexor tendon effectively after anastomonsis operation. The in situ hybridization examination revealed that TGF-β1 and Collagen Ⅰ mRNA expression in antisense TGF-β1 recombinant plasmid group was lower than that in normal saline group at each time point. Conclusion Antisense TGF-β1 recombinant plasmid can inhibit the function of the TGF-β1 effectively after the flexor tendon injured and repaired and prevent adhesion formatin.

关 键 词：肌腱 反义寡核苷酸类 TGF-Β1 胶原 

分 类 号：R687.2[医药卫生—骨科学]