HPLC测定不同产地决明子中蒽醌类成分  

Determination of anthraquinone in Semen Cassiae from different regions by HPLC

作　　者：张毅[1] 黄小平[1] 翁代群[1] 杨大坚[1,2] 

机构地区：[1]重庆市中药研究院,重庆400065 [2]香港理工大学现代中药研究所,深圳518057

出　　处：《中国中药杂志》2008年第23期2797-2799,共3页China Journal of Chinese Materia Medica

摘　　要：目的：测定不同产地决明子中蒽醌类成分的含量。方法：以Inertsil ODS-3为色谱柱，乙腈-0．1％磷酸水溶液为流动相，梯度流速0．8mL·min^-1，检测波长278nm，测定决明子药材中7个蒽醌类化学成分的含量。结果：各类成分的回收率均在95％～105％，含量因产地不同差异较大。结论：HPLC梯度洗脱法测定决明子药材中蒽醌类成分，方法简便、准确，为决明子质量评价和标准制定提供方法。Objective： To determine the content of 7 anthraquinones in Semen Cassiae. Method： A HPLC method was developed, with Inertsil ODS-3 column, acetonitrile and 0. 1% H3 PO4 solution as mobile phases in gradient elution. The detection wavelength wasset at 278 nm, and the flow rate was 0. 8 mL · min^-1. Result： Recoveries of all 7 anthraquinones were between 95%-105%. The content of the anthraquinones in crude drug produced in different habitation were different. Conclusion： The method is convenient and accurate, which provides the foundation for the research of Semen Cassiae.

关 键 词：决明子 HPLC 蒽醌 钝叶素 橙黄决明素 

分 类 号：R284.1[医药卫生—中药学]