刺五加抗疲劳活性部位中刺五加苷B的含量测定  

Determination of eleutheroside B in antifatigue fraction of Acanthopanax senticosus by HPLC

作　　者：李辰[1] 王晓燕[2] 胡绪玮[1] 范海涛[2] 乔善义[2] 

机构地区：[1]沈阳药科大学,辽宁沈阳110016 [2]军事医学科学院毒物药物研究所,北京100850

出　　处：《中国中药杂志》2008年第23期2800-2802,共3页China Journal of Chinese Materia Medica

基　　金：全军医药卫生科研基金课题B类(01MB316)

摘　　要：目的：建立刺五加抗疲劳活性部位中刺五加苷B的含量测定方法。方法：用小鼠负重游泳实验，确定刺五加粗提物的抗疲劳活性部位；硅胶柱色谱，Sephadex LH-20柱色谱，制备液相色谱对活性部位分离纯化，并对分离所得化合物进行理化性质研究和结构鉴定；含量测定中高效液相色谱条件为Diamonsil C18柱（4．6mm×250mm，5μm），流动相乙腈-水-醋酸（10：90：0．01），流速1．0mL·min^-1，紫外检测波长344nm。结果：从刺五加抗疲劳活性部位Asl中分离得到一化合物，鉴定为刺五加苷B；刺五加苷B的线性范围为0．104—20．8μg，r=0．9999；平均回收率为97．68％，RSD1．4％（n=6）。结论：从刺五加抗疲劳活性部位Asl中分离得到含量较高的刺五加苷B，作为质量控制的指标成分，并建立了刺五加抗疲劳活性部位中刺五加苷B含量测定方法。Objective： To develop an HPLC method for the determination of eleutheroside B in Asl （the extract fraction from Acanthopanax senticosus, which has a good effect of antifatigue）. Method： The antifatigue effect of Asl was evaluated by mice burden swimming testing. One compound was isolated by silica gel, Sephadex LH -20 column chromatography and preparative high performance liquid chromatography. The structure was identified by physicochemical properties and spectral evidences. Eleutheroside B in Asl was determined by HPLC. Chromatographic conditions included Diamonsil C18 column （4. 6 mm × 250 mm, 5 μm） and the mobile phase consisting of a mixture of acetonitrile-water-ethanoic acid （ 10： 90： 0. 01 ）. The UV detection wavelength was set at 344 nm. Resuit： Asl showed an excellent antifatigue activity; One compound was isolated from Asl and its structure was identified as Eleutheroside B; The calibration cure was linear in the range of 0. 104-20. 8μg （r =0. 999 9）, the average recovery was 97.68% , RSD 1.4% （ n = 6）. Conclusion ： This HPLC method is simple, accurate and reliable.

关 键 词：刺五加 抗疲劳 刺五加苷B 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]