杭白菊总黄酮对铅诱导小鼠氧化损伤的拮抗效应研究  

Antagonism of total flavonoids from Chrysanthemum morifolium against lead induced oxidative injury in mice

作　　者：夏道宗[1] 吕圭源[2] 于新芬[3] 王慧铭[1] 杨晴[1] 

机构地区：[1]浙江中医药大学药学院,浙江杭州310053 [2]浙江中医药大学药物研究所,浙江杭州310053 [3]杭州市疾病预防控制中心,浙江杭州310006

出　　处：《中国中药杂志》2008年第23期2803-2808,共6页China Journal of Chinese Materia Medica

基　　金：浙江省自然科学基金项目(Y207783);浙江省中医药优秀青年人才研究计划(2005Y006)

摘　　要：目的:探讨杭白菊总黄酮(total flavonoids from Chrysanthemum morifolium,TFCM)对铅诱导小鼠脑、肝脏和肾脏氧化损伤的拮抗效应。方法:将90只雄性小鼠分为9组:正常组,模型组,阳性药组,TFCM低、中、高(150,300,500 mg.kg-1)剂量组,TFCM低、中、高剂量+二巯基丁二酸(DMSA,70 mg.kg-1)组。前20 d,除正常组外,其余小鼠隔天腹腔注射醋酸铅建立铅中毒模型;接下来的10 d,各组小鼠灌胃给予受试物。实验结束后,摘眼球取血及各脏器,测定全血、脑、肝脏、肾脏中铅、锌和铜的含量,计算脏体比重系数,并对抗氧化酶活性及脂质过氧化水平进行检测和分析。结果:TFCM可拮抗铅中毒小鼠体重的下降和脏体比重的升高。单独使用TFCM对小鼠血液及组织中铅浓度并无显著影响,但与DMSA联合使用时排铅效果显著(P<0.01),且未造成锌、铜的流失。中、高剂量TFCM在增加小鼠脑GSH含量,GSH-Px,SOD,CAT活性及降低MDA含量方面,效果明显优于DMSA;高剂量TFCM在增加小鼠肝脏和肾脏GSH-Px,CAT活性及降低MDA含量方面,效果也明显优于DMSA。TFCM与DMSA联合使用时,也可显著改善小鼠脑、肝脏和肾脏脂质过氧化,增加GSH,GSH-Px,SOD和CAT的活性,效果优于单独使用DMSA。结论:TFCM可显著提高铅中毒小鼠组织中抗氧化酶的活性,改善脂质过氧化,从而明显拮抗铅诱导的脑、肝脏和肾脏氧化损伤。Objective： To investigate antagonism effects of total flavonoids from Chrysanthemum morifolium. （TFCM） against lead induced oxidative injury. Method： Ninety male mice were randomly divided into 9 groups. Mice except normal control group inject lead acetate every other day for 20 days. In the next 10 d, drugs were orally administrated to mice once a day. After the last aministration, mice were sacrificed and immediately subjected to necropsy. The concentration of lead, zinc and copper in blood, brain, liver and kidney were determined. The body weight, relative organ weight, antioxidant enzyme levels （GSH, GSH-Px, SOD and CAT） and lipid peroxidation products （MDA） were performed. Result： TFCM might antagonize the decrease of body weight and the increase of organ weight/body weight ratio. The combined treatment with TFCM and DMSA can significantly lower the lead levels in blood, brain, liver and kidney. In contrast, lead concentration in mice treated with TFCM alone did not show significant change in these organs. The other trace elements such as zinc and copper had no significant decrease after TFCM or DMSA treatment. Middle and high-dose TFCM was more effective than DMSA in increasing the activity of GSH, GSH-Px, SOD, CAT and decreasing the concentration of MDA in mice brain. In addition, high-dose TFCM was more effective than DMSA in increasing the activity of GSH-Px, CAT and decreasing the concentration of MDA in mice liver and kidney. The combined treatment with TFCM and DMSA also can reverse lipid peroxidation and increase antioxidant enzyme levels in lead poisoning mice dose-dependently, and it had more beneficial effects than treatment with DMSA alone. Conclusion： TFCM might improve antioxidant defense system, reverse lipid peroxidation and protect brain, liver and kidney against lead induced oxidative damage in mice significantly.

关 键 词：杭白菊 总黄酮 铅中毒 氧化损伤 药物拮抗效应 

分 类 号：R285.5[医药卫生—中药学]