γ-亚麻酸对实验性大鼠动脉粥样硬化形成初期的影响  

Effects of gamma linolenic acid on atherosclerosis induced by cholesterol-rich diet in rats

作　　者：施丽梅[1] 葛海涛[2] 孔秀芹[3] 蔡元锋[1] 李朋富[1] 刘志礼[1] 孔令东[1] 

机构地区：[1]南京大学生命科学院,江苏南京210093 [2]中国药科大学生药学教研室,江苏南京210038 [3]南京师范大学生命科学学院,江苏南京210046

出　　处：《中国中药杂志》2008年第23期2808-2812,共5页China Journal of Chinese Materia Medica

基　　金：黄河集团资助的研究课题

摘　　要：目的:探讨γ-亚麻酸(GLA)对实验性大鼠动脉粥样硬化形成初期的影响及其作用机制。方法:采用GLA作为受试物,将60只健康成年雄性SD大鼠,随机分为正常组、模型组、阳性药洛伐他汀组0.9 mg.kg-1.d-1、多烯康组250 mg.kg-1.d-1、GLA高、低剂量组(375,187.5 mg.kg-1.d-1)。光学显微镜检测HE染色的主动脉壁病理改变,测定TC,TG,HDL-C,LDL-C含量。结果:GLA可在一定程度上抑制高脂饲料诱导的SD大鼠主动脉血管壁病理变化,降低SD大鼠血清TC,TG,LDL-C水平,升高血清T-AOC活力,降低HL,LPL的含量,NOS活力降低,NO含量下降。结论:GLA可以有效调节血脂,改善血管病变,该作用与其调节脂蛋白脂酶、肝脂酶活性,影响NO及其合酶的形成和表达,提高机体总抗氧化能力相关。Objective： To study the effect of gamma linolenic acid （GLA） on atherogenesis in rats. Method： Sixty healthy male rats were randomly divided into 6 groups ： normal contro 1, fed by normal feed ; atherogenesis mode 1, fed by high lipid diet; positive control group 0. 9 mg ·kg^-1·d^-1 of lovastatin and group Ⅳ 250 mg ·kg^-1·d^-1 duoxikang; high dose of 375 mg·kg^-1·d^-1 GLA; low dose of 187.5 mg·kg^-1·d^-1 GLA. After the model group received atherogenic diet for six weeks, serum triglyceride （TG）, total cholesterol （TC）, low density lipoprotein cholesterol （LDL-C） and high density lipoprotein cholesterol （HDL-C） were detected by enzyme method to confirm the formation of atherogenic. After fed for another five weeks, morphologic atherosclerosis of aorta in rats was observed by HE staining methods. The blood samples were collected and serum TC, TG, HDL-C, LDL-C, T-AOC, HL, LPL, NO, NOS, MDA and GSH were determined. Result： GLA attenuated the formation of atherosclerotic plaques, inhibited the level of serum TC, TG, MDA, OX-LDL, NO, NOS, HL, LPL and LDL-C and increased the level of T-AOC. Conclusion： GLA might significantly attenuate the development of atherosclerosis in rats fed with high lipid diet through improving the antioxidation capacity of the body.

关 键 词：Γ-亚麻酸 动脉粥样硬化 脂酶 一氧化氮 抗氧化 

分 类 号：R285.5[医药卫生—中药学]