大鼠脑纹状体微透析法研究天王补心方剂中桔梗的应用与抑制性神经递质变化的相关性  

Research for dependablity of administration of platycodi radix in Tianwang Buxinwan decoction with change of brain inhibitive neurotransmitte in rats by microdialysis

作　　者：刘萍[1] 何新荣[1] 周文斌[2] 沈然然[3] 冯帆[4] 

机构地区：[1]解放军总医院药品保障中心中药房,北京100853 [2]解放军军事医学科学院二所九室,北京100850 [3]安徽蚌埠医学院药学院,安徽蚌埠233000 [4]北京联合大学生化学院,北京100023

出　　处：《中国中药杂志》2008年第23期2830-2833,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30472202)

摘　　要：目的:研究天王补心丸对大鼠脑内氨基酸类神经递质的影响,来探讨该药的安神作用机制。方法:利用微透析技术从给药大鼠脑纹状体取样,利用邻苯二甲醛(OPA)柱前衍生反相高效液相色谱法结合荧光检测器测定标准品和样品中抑制性氨基酸(甘氨酸、γ-氨基丁酸)的含量并进行统计分析。结果:全方组、桔梗+酸枣仁组给药后大鼠纹状体区细胞外液中兴奋性氨基酸含量变化不明显,而抑制性氨基酸GABA和Gly含量均有所增加。结论:本方法具有快速、准确、灵敏度高、稳定性好等优点,适用于脑组织中微量氨基酸类神经递质的测定。桔梗对大鼠脑中抑制性神经递质有明显的影响。Objective： To study the effects of Tianwang Buxinwan decoction on the contents of amino acids neurotransmitters in corpus striatum of rats to implicate the mechanism of Tianwang Buxinwan promoting and Improving sleeping. Method： Contents of two amino acids neurotransmitters in corpus striatum of rats were prepared by microdialysis technology and were determined by HPLC which involved pre-column derivation with orthophthaladehyde, recersed-phase gradient elution and fluorescence detection. Result： In the experimental separation condition, Tianwang Buxinwan seemed do not influence three kinds of contents of amino acids neurotransmiters （glutamic acid, glycin, aspartic acid）, but TBW seemed increase the content of γ-GABA in corpus striatum of rats. Conclusion： The effects of Tianwang Buxinwan to relieve uneasiness may relate with the inhibitory amino acids γ-GABA. Tianwang Buxinwan may promote increasing the content of γ-GABA. This discovery may be helpful for the deep study of related mechanism of Tianwang Buxinwan.

关 键 词：天王补心丸 微透析 神经递质 桔梗 舟楫作用 

分 类 号：R285.5[医药卫生—中药学]