野菊花总黄酮对佐剂性关节炎大鼠滑膜细胞的凋亡诱导作用  

Beneficial effect of total flavonoids of Chrysanthemum indicum on adjuvant arthritis by induction of apoptosis of synovial fibroblasts

作　　者：解雪峰[1] 李俊[1] 陈镇[2] 胡成穆[1] 陈伟薇[1] 

机构地区：[1]安徽医科大学药学院,安徽合肥230032 [2]安徽医科大学第一附属医院药剂科,安徽合肥230032

出　　处：《中国中药杂志》2008年第23期2838-2841,共4页China Journal of Chinese Materia Medica

基　　金：国家科技部重大基础研究项目(2002CCCC02900);安徽医科大学校科研基金项目(2006kj20)

摘　　要：目的:研究野菊花总黄酮(TFC)对佐剂性关节炎(AA)大鼠滑膜细胞的凋亡诱导作用,探讨TFC治疗AA的可能机制。方法:SD大鼠随机分成6组:正常组、模型组、TFC高、中、低剂量组和阳性药雷公藤多苷(TPT)组;SD大鼠右后足跖皮内注射弗氏完全佐剂复制AA模型;MTT法检测滑膜细胞的增殖;原位末端标记检测(TUNEL)法观察滑膜组织中滑膜细胞的凋亡改变;流式细胞仪检测体外培养滑膜细胞的凋亡变化。结果:AA大鼠滑膜细胞出现凋亡抑制,增殖过度的类瘤样细胞特征,TFC可剂量依赖性地降低AA大鼠滑膜细胞的炎性增殖;TFC(168,336 mg.kg-1)治疗组大鼠滑膜细胞的凋亡率较模型组显著升高。结论:TFC可抑制AA大鼠滑膜细胞的过度增殖,诱导滑膜细胞凋亡,这可能是其治疗AA的机制之一。Objective： To investigate the effect on the extract of total flavonoids of Chrysanthemum indicum （TFC） on adjuvant arthritis synovial cells. Method： SD rats were divided randomly into six groups including normal, model, TFC （84, 168,336 mg · kg^-1 ） and control drug Tripterygium glycosides （30 mg· kg^-1 ） groups. Adjuvant arthritis rat model was induced by a single intradermal injection of 0. 1 mL of the complete Freund＇s adjuvant into the right hind feet pads of the SD rats. The proliferation of synoviocyte was measured by MTT; The apoptosis rates of synovial cells were evaluated using TUNEL and FCM analysis. Result： TFC resulted in a dose-dependent way in inhibiting the proliferation of synovial and inducing the apoptosis of synovium and synoviocytes in vivo. Conclusion： TFC can inhibit proliferation and induce apoptosis in synovial cells, and exert therapeutical effect on rheumatoid arthritis.

关 键 词：野菊花总黄酮 佐剂性关节炎 细胞凋亡 原位末端标记检测法 流式细胞术 

分 类 号：R285.5[医药卫生—中药学]