红景天对慢性应激导致的抑郁大鼠大脑海马5-羟色胺水平及其细胞增殖、分化和神经元数量的影响  

Effects of Rhodiola rosea on level of 5-hydroxytryptamine,cell proliferation and differentiation,and number of neuron in cerebral hippocampus of rats with depression induced by chronic mild stress

作　　者：秦亚静[1] 曾园山[1] 周春春[1] 李燕[1] 钟志强[1] 

机构地区：[1]中山大学中山医学院组织胚胎学教研室神经科学研究室,广东广州510080

出　　处：《中国中药杂志》2008年第23期2842-2846,共5页China Journal of Chinese Materia Medica

摘　　要：目的:探讨中药红景天Rhodiola rosea对慢性应激导致的抑郁大鼠大脑海马5-羟色胺(5-HT)水平及其细胞增殖、分化和神经元数量的影响。方法:50只大鼠被分为正常对照组、模型组、阴性对照组、阳性对照组及红景天治疗组,每组各10只动物。除正常对照组外,给予其余4组大鼠为期4周的慢性应激,以建立抑郁症模型。在模型建立后,除模型组正常饲养外,其余4组大鼠分别灌服5%羧甲基纤维素钠、氟西汀、红景天,灌药周期均为3周。灌药结束后,采用高效液相色谱法检测海马组织5-HT水平,5-溴脱氧尿嘧啶(BrdU)和β-微管蛋白Ⅲ(β-tubu-linⅢ)免疫荧光组化双标记染色检测海马神经干细胞的增殖和分化、采取形态计量学方法计数海马神经元。结果:与正常对照组比较,红景天治疗组的大脑海马5-HT水平、BrdU标记细胞数量、β-tubulin III和BrdU双标记细胞百分率以及神经元数量恢复性增加到正常对照组大鼠的水平。结论:红景天能够提高慢性应激导致的抑郁大鼠大脑海马5-HT水平,促进其海马神经干细胞增殖和分化,同时具有保护受损伤的海马神经元的作用。Objective： To explore the effects of Rhodiola rosea on the level of 5-hydroxytryptamine （5-HT）, cell proliferation and differentiation, and number of neuron in cerebral hippocampus of rats with depression induced by chronic mild stress. Method： Fifty rats were divided into 5 groups： normal control, untreated, negative control, positive control and Rhodiola rosea-treated groups. There were 10 rats in each group. Except for normal control group, depression was induced in rats by chronic mild stress. The depressive rats in the other four groups were intragastrically administered with 0. 5% sodium carboxymethycellulose, fluoxctine and Rhodiola rosea for 3 weeks. After the treatment, the content of 5-HT in the hippocampus was detected by high-performance liquid chromatography. The proliferating cells and differentiated cells in the hippocampus were labeled by bromodeoxyuridine （BrdU） or/and β-tubulin Ⅲ immunohistochemistry, and the number of hippocampal neurons was counted by morphometry. Result： Compared with the normal control group, the content of 5-HT, number of BrdU positive cells, percentage of BrdU and β-tubulin Ⅲ double labeled cells and number of neurons in cerebral hippocampus in the Rhodiola rosea-treated group were increased and recovered to normal level. ConclusiOn： Rhodiola rosea may enhance the level of 5-HT and promote the proliferation and differentiation of neural stem cells in the hippocampus of the depressive rats, and may play a role in saving injured neurons of the hippocampus.

关 键 词：红景天 抑郁症 海马 神经干细胞 分化 

分 类 号：R285.5[医药卫生—中药学]