高效液相色谱法测定水体中的氯虫酰胺残留量  

Determination of Chlorantraniliprole Residue in Environmental Aquatic Samples by High Performance Liquid Chromatography

作　　者：步海燕[1] 欧晓明[2] 马俊凯[1] 蔡德玲[3] 

机构地区：[1]华中师范大学化学学院,武汉市珞瑜路100号430079 [2]湖南化工研究院国家农药创制工程技术研究中心,长沙市芙蓉中路二段251号410007 [3]中南大学化学化工学院,长沙市岳麓区麓山南路410083

出　　处：《光谱实验室》2008年第6期1230-1234,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：湖南省自然科学基金项目(编号06JJ20054)

摘　　要：采用Kromasil C18不锈钢柱(250mm×4.6mm i.d.,5μm),以甲醇-水混合溶剂(65∶35,V/V)为流动相,流速1.0mL/min,光电二极管阵列检测器,检测波长236nm和柱温35℃,建立了不同水体中氯虫酰胺残留的高效液相色谱分析方法。氯虫酰胺水样直接用二氯甲烷进行液-液分配萃取。在0.10—10mg/L的浓度范围内,氯虫酰胺峰面积(y)与质量浓度(x)呈现良好的相关性,其线性回归方程为y=2170.4x+2641.7,r2=0.9991。井水和池塘水中添加质量浓度为0.01—1.00mg/L时,其平均回收率分别为94.70%—100.37%和89.14%—100.22%,变异系数分别为0.81%—5.11%和1.81%—5.30%,水样中的氯虫酰胺最低检测质量浓度(LOQ)为7.5×10-3mg/L,方法快速、灵敏度高、重现性好,具有较好的准确度和精密度,可用于环境中水样和土壤样中氯虫酰胺的残留检测分析。Chlorantraniliprole residues in the environmental aquatic system was extracted by liquid-liquid partition from water samples into dichloromethane,and determined by high performance liquid chromatography.The analysis was performed on a Kromasil C 18 stainless steel column（250mm×4.6mm i.d.,5μm） with mixture of methanol-water（65∶35,V/V）as the mobile phase at a flow rate of 1.0mL/min,at the column temperature of 35℃ and photodiode array detector at 236nm.The calibration curve for chlorantraniliprole showed a good linearity in the concentration range of 0.10—10mg/L with the regression equation y=2170.4x＋2641.7,r2=0.9991.The average recovery of five spiked concentration levels for chlorantraniliprole in well water and pond water were 94.70%？ ？00.37% and 89.14%—100.22%,respectively.The coefficient of variation（CV） was 0.81%？ ？.11% and 1.81%—5.30%,respectively.The limit of quantitation（LOQ） for chlorantraniliprole was 7.5×10 -3 mg/L.The method was fast,sensitive,simple,reproducible and practical for the residue detection and analysis of chlorantraniliprole in aquatic systems.

关 键 词：氯虫酰胺 水样 残留分析 高效液相色谱 

分 类 号：S482.3[农业科学—农药学] O657.72[农业科学—植物保护]