γ-羟丁酸钠对肝脏缺血再灌注损伤的保护作用  

Protective Effects of Sodium Hydro Butyrate on Liver Ischemic Reperfusion Injury in Rats

作　　者：刘欣[1] 莫坚[1] 曹殿青[1] 薛晓英[1] 何海燕[1] 

机构地区：[1]广东医学院附属医院麻醉科,广东湛江524001

出　　处：《湖北民族学院学报（医学版）》2008年第4期21-23,共3页Journal of Hubei Minzu University(Medical Edition)

摘　　要：目的观察γ-羟丁酸钠对大鼠肝脏缺血再灌注损伤中的保护作用及其可能机制。方法36只雄性Wist-ar大鼠随机分成3组,每组12只。假手术组:开腹显露第一肝门后关腹;缺血再灌注组:开腹显露第一肝门后,用无损伤血管钳夹闭肝蒂,阻断血流30 min后,放开血管钳再灌注2 h,制作常温下大鼠肝脏热缺血模型;γ-羟丁酸钠处理组:麻醉后经静脉泵注γ-羟丁酸钠60 mg/kg,余同缺血再灌注组。分别于缺血再灌注6 h后抽血检测血清AST、ALT、SOD及MDA水平,并取肝组织行病理组织学检测和Westernblot法检测HMGB-1水平。结果缺血再灌注组AST、ALT、MDA及HMGB-1的含量均高于假手术组和γ-羟丁酸钠组(P<0.01)。而γ-羟丁酸钠组SOD的活性高于缺血再灌注组(P<0.05)。结论γ-羟丁酸钠预处理对肝脏缺血再灌注损伤具有明显的保护作用。Objective To study the protective effects of sodium hydrobutyrate on liver ischemic reperfusion injury and its mechanism in rats. Methods 36 healthy male Wistar rats were randomized into three groups： Control group （C group）, ischemic reperfusion group （IR group） and ischemic hydrobutyrate preconditioning group （P group）, each consisting of 12 animals. IR group and P group were made liver ischemic reperfusion injury model, and P group were pretreated with sodium hydrobutyrate 60 mg/kg, The plasma ALT, AST, SOD and MDA levels were measured, and HMGB -lin the liver tissue were also observed using western blot methods. Results The plasma ALT,AST and MDA levels in IR group were significantly higher than those in C group and P group（P 〈0.01） ,and SOD levels in IR group was higher than that in C group and P group（P 〈0.05）. HMGB - 1 had no expression in C group, but its mRNA was higher in IR group than in P group（P 〈0.01 ）. Conclusion With the sodium hydrobutyrate pretreatment, the liver tissue was protected efficiently against ischemic reperfusion injury.

关 键 词：Γ-羟丁酸钠 HMGB-1 缺血再灌注损伤 

分 类 号：R969.4[医药卫生—药理学]