肠型点状气单胞菌口服疫苗微粒的制备及体外释放研究  

Preparation and in Vitro Release of Oral Vaccine Microsphere against Bacterium Aeromonas punctata f.intestinalis

作　　者：孙翰昌[1] 杨帆[1] 

机构地区：[1]重庆文理学院生命科学系,重庆永川402168

出　　处：《水产科学》2008年第12期658-661,共4页Fisheries Science

基　　金：重庆市自然科学基金资助项目(CSTC;2007BB1373);重庆文理学院校内科研项目(Z2007SK12)

摘　　要：采用复乳溶剂挥发法,以聚丙烯酸树脂Ⅲ为材料制备肠型点状产气单胞菌疫苗微球,进行了条件优化。研究结果显示,制备得到的口服疫苗微粒形态良好,平均粒径(6.78±0.07)μm,载药量和包封率分别为(6.04±0.07)%和(63.52±0.06)%,19 d时的累积体外释放百分率为65%。复乳溶剂挥发法置备的肠型点状产气单胞菌疫苗微球工艺可行,重复性良好,有一定的缓释特性。Oral vaccine （ bacterium Aeromonas punctata f intestinalis） microspheres were prepared by double emulsion in liquid drying process using polyacrylic resin Ⅲ as carrier and the preparative condition including the drug release in vitro was also optimized. The results showed that the drug loading and entrapment efficiency were （6.04 ± 0.07） % and （63.52 ± 0.06 ） %, mean particle size of （6.78 ± 0.07 ） μm. Under the release condition in vitro, accumulative drug release of vaccine microspheres was 65% within 19 d. The bacterium encapsulated in the microspheres using biodegradable Polyacrylic resin Ⅲ as carrier supported to yield haft-month sustained release.

关 键 词：肠型点状产气单胞菌 口服疫苗微粒 体外释放 

分 类 号：S965.112[农业科学—水产养殖]