二苯并噻吩生物降解菌培养和反应条件的优化  

Optimization on cultivation and reaction conditions of biodegradation bacterium of dibenzothiophene

作　　者：侯影飞[1,2] 孔瑛[2] 郭宁[2] 祝威[1] 张建[1] 

机构地区：[1]中国石化胜利工程设计咨询有限责任公司,山东东营257026 [2]中国石油大学重质油国家重点实验室,山东东营257061

出　　处：《中国石油大学学报（自然科学版）》2008年第6期138-141,共4页Journal of China University of Petroleum(Edition of Natural Science)

基　　金：国家自然科学基金项目(20676128)

摘　　要：考察了培养温度、时间、pH值对根癌土壤杆菌UP-3生长的影响,选择了最合适的培养条件。通过考察底物浓度、反应温度和反应时间对菌株UP-3降解二苯并噻吩(DBT)性能的影响,优化了生物降解的反应条件。结果表明:UP-3最佳培养条件为培养温度31℃,培养时间30 h,培养基pH值7.3;UP-3催化降解DBT反应的适宜条件为DBT质量浓度500 mg/L,反应温度31℃,反应时间35 h;适宜条件下UP-3生长细胞对DBT的降解率达到85%,说明其具有较好的应用潜力。Temperature, time and pH value of cultivation on growth of Agrobacterium tumefaciens UP-3 （ CGMCC NO. 0973） were studied, and the suitable conditions were selected. The influences of substrate concentration, temperature and time of reaction on degradation of dibenzothiophene（DBT） with Agrobacterium tumefaciens UP-3 were analyzed. And the conditons of degradation reaction were optimized. The optimal cultivation conditions are as follows： Temperature is 31 ℃, time is 30 hours, and pH is 7.3. The degradation reaction of DBT would be better carried out at the temperature of 31℃ with the reaction time of 35 hours and the DBT concentration of 500 mg/L. On the suitable conditions, growing cells of UP-3 can degrade 85% of 500 mg/L DBT. As a result, the UP-3 is one kind of the potential bacteria for biodesulfurization.

关 键 词：生物脱硫 二苯并噻吩 生长细胞 根癌土壤杆菌UP-3 

分 类 号：Q819[生物学—生物工程]