中药的毛细管电泳指纹图谱的研究方法  

Research method for capillary electrophoresis fingerprints of traditional Chinese medicines

作　　者：孙国祥[1] 宋文璟[1] 宋杨[1] 王真[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《中南药学》2008年第6期752-757,共6页Central South Pharmacy

基　　金：国家自然科学基金重大研究计划项目(No.90612002)

摘　　要：目的介绍了毛细管电泳指纹图谱的优势,毛细管电泳指纹图谱的研究方法与评价方法及其在中药质量控制中的应用。方法以中药指纹图谱质量控制特征为基础,综述了毛细管电泳在中药指纹图谱方面应用的基础理论和方法。结果建立了一套完整的中药指纹图谱研究方法,包括实验条件的优化、系统适用性试验、电泳峰的指认与标定、双定性双定量相似度评价法等。结论数字化指纹图谱技术是数字中药质控的典型代表和核心技术,建立毛细管电泳数字化指纹图谱是实现中药现代质量控制的重要技术之一。Objective The advantages of CEFP were introduced, and the research and appraisal method of CEFP for traditional Chinese medicine were also discussed. Methods To review the basic theories and methods of capillary electrophoresis fingerprint （CEFP） of traditional Chinese medicines based on quality controlled characteristics of traditional Chinese medicine fingerprints. Results A complete set of research methods was set up, including the optimum experimental Conditions of CE, system suitability, identification and calibration of electrophoresis peaks, and dual qualitative and dual quantitative similarities. Conclusion Digitized fingerprint is typical representative and core technology in the quality control of traditional Chinese medicine, and the establishment of CEFP is one of the important technologies to realize modern quality control for traditional Chinese medicine.

关 键 词：毛细管电泳 数字化指纹图谱 中药 毛细管电泳-质谱联用 

分 类 号：R284[医药卫生—中药学] O234[医药卫生—中医学]