大气混合污染物对大鼠肺泡灌洗液及血清中CC16表达水平的影响  

Effects of air mixed certain pollutants on the expression of CC16 in BALF and serum of rats

作　　者：陈魁敏[1] 王任群[2] 赵肃[2] 肖纯凌[3] 

机构地区：[1]沈阳医学院机能实验中心,辽宁沈阳110034 [2]沈阳医学院职业卫生与环境卫生学教研室,辽宁沈阳110034 [3]沈阳医学院病原生物学教研室,辽宁沈阳110034

出　　处：《沈阳医学院学报》2008年第4期195-197,共3页Journal of Shenyang Medical College

基　　金：国家自然科学基金资助项目(No.30471425)

摘　　要：目的:探讨大气混合污染物所致肺损伤的敏感生物指示物。方法:使Wistar大鼠染尘、染毒,制备大气混合污染物所致肺损伤的动物模型。将48只Wistar大鼠随机分为3个实验组(1d组、15d组、30d组)和3个对照组,每组8只。采用ELISA方法检测肺支气管肺灌洗液(BALF)和血清中CC16蛋白的表达。结果:BALF中,染毒ld组CC16蛋白水平降低,与对照组比较差异显著(P<0.05);血清中,染毒30d大鼠CC16蛋白与对照组比较显著升高(P<0.05)结论:CC16可作为急性肺损伤的敏感生物指示物之一。Objective： To determine CC16 in BALF and serum of rats exposed to air mixed pollutants on lung damage. Methods： 48 Wistar rats with 200 -240g weight were randomly divided into 3 experimental groups （1d , 15d, 30d dose group） and 3 control groups. Each group had 8 rats. ELISA was utilized to measure CC16 level in BALF and serum. Results： The level of CC16 in BALF of ld experimentalwas lower than that of control group （p 〈 0. 05 ）. CC16 level in serum was hight than that of control group （P 〈0. 05）. Conclusion： The level of CC16 may be used as a peripheral biomarker in the acute lung injury.

关 键 词：大气混合污染物 BALF 血清 CC16 

分 类 号：R562.25[医药卫生—呼吸系统] R322.85[医药卫生—内科学]