电离辐射致DNA双链断裂研究方法及统计模型  

Detecting methods and statistical models in studying on DNA double-strand breaks induced by ionizing radiation

作　　者：周莉薇[1] 陈晓明[1] 张建国[1] 柳芳[1] 

机构地区：[1]西南科技大学生命科学与工程学院,绵阳621010

出　　处：《辐射研究与辐射工艺学报》2008年第6期321-325,共5页Journal of Radiation Research and Radiation Processing

基　　金：西南科技大学核废物与环境安全国防重点学科实验室基金(07JGZB07);中国工程物理研究院预研基金(052056);中国工程物理研究院核物理与化学研究所资金(07zh0132)资助

摘　　要：DNA既是生命物质中信息的载体,也是辐射生物效应最主要的靶分子。电离辐射通过射线的直接作用和间接作用引起DNA分子的多类型损伤,其中DNA双链断裂(Double-strandbreaks,DSB)是辐射引起的各种生物效应中最重要的原初损伤之一,成为辐射生物学研究的重点。目前DSB研究的主要方法包括拉曼光谱技术、原子力显微镜、单细胞凝胶电泳、脉冲场凝胶电泳、γH2AX分析技术、早熟染色体凝集等,研究DSB的统计模型包括随机断裂模型、Moment法、Tsallis熵模型、平均分子量法,在此均得到总结,最后探讨了DSB辐照热点的研究前景。A DNA molecule is both the carder of biological information and the main target of biological effects induced by ionizing radiation. Irradiation leads to many types of DNA damage by the direct and the indirect radiation effect. DNA double-strand breaks （DSB） induced by ionizing radiation represent the primary damage of all bio-effects. Currently, the detecting methods dealing with DSB include raman spectroscopy analysis, atomic force microscopy analysis, single cell gel electrophoresis, pulsed-field gel electrophoresis, γH2AX analysis and premature chromosome condensation analysis, etc. And the statistical models of DNA DSB involve in the random fracture model, the moment method, the tsallis entropy model and the average molecular weight method. Both of them have been discussed in this paper. Finally, the research prospect of the DNA DSB radiation hotspot is presented.

关 键 词：DNA双链断裂 双链断裂研究方法 双链断裂统计模型 双链断裂辐照热点 

分 类 号：O056[理学] Q6[生物学—生物物理学]