切尔诺贝利事故清理工作者淋巴细胞的尾核  

Tailed nuclei in lymphocytes from Chernobyl liquidators

作　　者：李雨[1] 李智恒[1] V.Yu.Kravtsov S.S.Aleksanin 

机构地区：[1]第二军医大学海军医学系放射医学教研室 [2]All-Russia Center of Emergency and Radiation Medicine,194044 St.Petersburg,Russia

出　　处：《辐射研究与辐射工艺学报》2008年第6期371-373,共3页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金面上项目"切尔诺贝利核事故清理工作人员受照远后基因表达变化"(30270421)资助

摘　　要：为了研究切尔诺贝利事故清理工作者和其他受照射人员外周血淋巴细胞,选取切尔诺贝利事故清理工作者和其他受照射人员作为试验组,采用常规方法取指尖外周血制备涂片,空气干燥,对氨基苯甲酸混合,Giemsa法染色,光镜下计数每人500个细胞样本,观察记录具有尾状突起细胞核发生率,进行统计学处理。未照射对照组的外周血淋巴细胞尾核发生率在0%～0.6%,平均值0.16。而大多数受照射对象都出现较多具有尾核的淋巴细胞。照射组的淋巴细胞出现尾核最大值是3.2%,平均值是0.50%。切尔诺贝利事故清理工作者的带尾核的淋巴细胞出现的几率显著高于对照组(p<0.01)。外周血淋巴细胞尾核可能是一种有意义的受照射标记物,受照射淋巴细胞尾核的异常出现可能起因于双着丝粒所形成的染色体桥的断裂。对于更加详尽地获取辐射生物效应信息,回顾性研究辐射剂量效应可能具有重要的理论和实践价值。In order to study ＂tailed nuclei＂ （TN） in peripheral blood smears from irradiated Chemobyl liquida- tors, human peripheral blood lymphocytes from irradiated Chemobyl liquidators as experiment group were selected to observe under microscope and record different TN pattern in routine Giemsa dyeing method with 500 cells. Result shows that TN percent in control group is 0 to 1.0% while the TN percent in the irradiated group is higher （most is 3.2%）. The frequency of appearance of lymphocytes with TN in a group of Chemobyl liquidators is significandy higher than that in control groups （p〈0.01）. Abnormalities of the TN type in irradiated lymphocytes may be from breakdown of chromosome bridges formed in dicentrics. The TN could be considered as a possible marker involved in radiation damage of biological tissue.

关 键 词：切尔诺贝利核事故 辐射 尾核 

分 类 号：Q691.5[生物学—生物物理学]