HPLC测定戊己丸中盐酸小檗碱含量  

Determination of Berberine Hydrochloride in Wuji Pills by HPLC

作　　者：凌丽美[1] 汪艳[1] 宣自华[2] 

机构地区：[1]安徽省黄山市药品检验所,安徽黄山245000 [2]安徽中医学院

出　　处：《安徽中医学院学报》2008年第6期52-53,共2页Journal of Anhui Traditional Chinese Medical College

摘　　要：目的:建立戊己丸中盐酸小檗碱的含量测定法。方法:采用HPLC测定含量,色谱柱为Gemini C18(4.6 mm×250 mm,5μm),流动相为乙-腈0.05 mol/L磷酸二氢钾(100 g/L氢氧化钾溶液调pH 5.0,35∶65),检测波长为246 nm,流速为1.0 ml/min;柱温为25℃。结果:盐酸小檗碱分离完全,加样回收率为97.82%,RSD为0.80%。结论:HPLC测定戊己丸中盐酸小檗碱含量简便、快速而准确。Objective,To establish a method for the determination of berberine hydrochloride in Wuji Pills. Methods： The determination was performed by high performance liquid chromatography （HPLC）. The chromatographic column was Gemini C18 （4.6 mm× 250 mm,5μm）. The mobile phase was Acetonitrile-0.05 mol/L KH2PO5 （35 ： 65, pH5. 0）. The wave lenghth was 246 nm. The flow rate was 1. 0 ml/min. The column temperature was 25℃. Resuhs：Berberine hydrochloride was separated completely. The mean recovery rate was 97.82% and RSD was 0.80%. Conclusion：This method is simple, rapid and accurate.

关 键 词：戊己丸 盐酸小檗碱 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]