广西瑶、侗、苗、壮等民族外周血白细胞中BK病毒的研究  

Prevalence of BK Virus Infection in Guangxi Minority Nationalities

作　　者：龙柳艳[1] 

机构地区：[1]广西医科大学第四附属医院,广西柳州545005

出　　处：《实用预防医学》2008年第6期1719-1721,共3页Practical Preventive Medicine

基　　金：柳州市科技三项经费资助项目(柳科字2007021317)

摘　　要：目的了解广西瑶、侗、苗、壮等少数民族外周血白细胞中多瘤病毒BKV的感染情况,掌握该区人群BKV的携带情况,阐明BKV在人体内的传播方式。方法于2006年1月-2008年5月采集广西瑶、侗、苗、壮等少数民族及汉族健康人外周血标本各2500份,提取淋巴细胞基因组DNA,用巢式PCR方法扩增BKV的保守区编码序列,统计分析不同少数民族、年龄、性别组BKV-DNA检出率。结果2500份健康人外周血白细胞(PBLs)样品中BKV-DNA序列检出率为60.6%,不同民族、性别、年龄组之间无显著性差异(P>0.05)。结论BKV在广西瑶、侗、苗、壮等少数民族及汉族健康人PBLs中存在较高的感染率,本研究证实了PBLs是BKV在体内的潜伏细胞及传播载体,应加强对BKV条件致病性的认识,积极防治、减少BKV相关性肾病(BKVN)的发生率。Objective To investigate the prevalence of BK virus in peripheral blood leukocytes （PBLs） of healthy individuals from different minorities including Yao, Dong, Miao, Zhuang, and so on in Guangxi, and to describe the transmission of BKV in human body. Methods DNA in peripheral blood leukooytes from 2,500 blood samples of healthy adult individuals between January, 2006 and May, 2008 was extracted and BKV DNA was examined by nested polymerase chain reaction. The BKV- DNA detection rates were statistically analyzed among the groups of different minorities, ages and sex. Results In the 2,500 peripheral leukocytes samples, the detection rate of BKV- DNA was 60.6%. There was no significant difference in nationalities, sex or age distribution （P〉0.05）. Coaclusions It shows a high prevalence of BKV in peripheral blood leu- kocytes of healthy individuals from different minorities including Yao, Dong, Miao, Zhuang, and so on in Guangxi. This study shows that PBLs are the harbors and the transmission carriers for BKV. Awareness of the opportunistic pathogenicity of BKV should be improved so as to decrease and prevent the incidence of BKV nephrosis.

关 键 词：多瘤病毒BK 外周血自细胞 感染率 广西少数民族 

分 类 号：R373[医药卫生—病原生物学]