系统性红斑狼疮肾炎患者血清AnuA、抗dsDNA抗体检测的临床意义  

Clinical Significance of Combined Detection of Serum Anti-nucleosome and Anti-dsDNA antibodies in Patients With Lupus Nephritis

作　　者：郭婧婧[1] 刘建华[2] 

机构地区：[1]中南大学湘雅医院检验科,湖南长沙410008 [2]中南大学湘雅医学院检验系

出　　处：《实用预防医学》2008年第6期1931-1932,共2页Practical Preventive Medicine

摘　　要：目的探讨系统性红斑狼疮患者血清抗核小体抗体(AnuA)、抗dsDNA抗体检测的临床意义。方法分别采用酶联免疫吸附法(ELISA)、胶体金免疫渗滤法对108例系统性红斑狼疮(其中51例系统性红斑狼疮肾炎和57例系统性红斑狼疮无肾炎)患者血清进行AnuA、抗dsDNA抗体检测。结果51例系统性红斑狼疮肾炎(LN)患者,An-uA阳性30例,阳性率58.82%,抗dsDNA抗体阳性36例,阳性率70.58%。而57例SLE无肾炎患者组中,两者的阳性率分别为21.05%、31.58%。AnuA+抗dsDNA抗体联合检测阳性率为86.27%。结论血清AnuA、抗dsDNA抗体与LN的发生密切相关,联合检测这两种抗体对LN的诊断和病情监测有重要意义。Objective To investigate the clinical significance of anti - nucleosome antibody （AnuA） and anti - dsDNA antibody in systemic lupus erythematosus patients. Methods Enzyme- linked immunosorbent assay（ELISA）and colloidal gold immuno - percolation method were used to measure serum AnuA and anti - dsDNA in 108 patients with systemic lupus erythematosus （including 51 LN patients and 57 SLE patients without nephritis）. Results Of the 51 LN patients, 58.82% were seropositive for AnuA, and 70.58% were seropositive for antidsDNA. Of the 57 SLE patients without nephritis, the positive rates of the antibodies were 21,0.5 % and 31..58 % respectively. The positive rates of AnuA and anti - dsDNA in LN were significantly higher ihan those in SLE without nephritis （P〈 0.05）. Conclusions Serum AnuA and anti- dsDNA anti- bodies have a close relationship with the incidence of LN. Combined determination of AnuA and anti - dsDNA antibodies is of great value to diagnosis and monitoring illness conditions of LN patients.

关 键 词：抗核小体抗体 抗双链DNA抗体 狼疮肾炎 红斑狼疮 

分 类 号：R593.24[医药卫生—内科学]