低能N^＋注入油脂降解菌株的诱变选育及降解条件的初步研究  

Screening of an oil-degrading strain by N^+ implantation and the oil degradation conditions

作　　者：闫亚娟[1,2] 李宗伟[1] 刘建玲[2] 秦广雍[1] 

机构地区：[1]郑州大学离子束生物工程省重点实验室,郑州450052 [2]西北大学生命科学学院,西安710069

出　　处：《核技术》2008年第12期915-919,共5页Nuclear Techniques

基　　金：国家重点基础研究发展规划973项目(2004CB719604);农业部公益性行业科研专项项目(200803034)资助

摘　　要：通过N+注入解脂耶罗威亚酵母(Yarrowia lipolytica)DC-3-2,筛选出一株油脂降解率提高了11.09%的高效菌株DC-3-2-50,经10次传代实验表明该菌株遗传稳定向良好。对DC-3-2-50的降解条件进行了初步研究,结果表明,在最佳条件:初始pH9.0、接种量3.0%、温度为25~28℃、摇床转速为180~200rpm、碳源为大豆油时,其降解率最高,可达87.7%。A strain DC-3-2-50 was obtained through N^＋ implanting into Yarrowia lipolytica DC-3-2. An increase of 11.09% in the oil-degradation rate was obtained. The stain has good genetic stability after 10 times of subculture. The culturing condition of DC-3-2-50 was studied. The optimal culture conditions were as follow： initial pH value, 9.0; inoculum size, 3%; temperature, 25--28℃; dissolved oxygen, 180-200 rpm; and carbon nutriments soybean salad oil. The oH-degradation rate can be up to 87.7%.

关 键 词：N^+注入 解脂耶罗威亚酵母 接种量 溶氧量 

分 类 号：Q933[生物学—微生物学]