磷酸苯丙哌林与曙红B的相互作用及其分析应用  

Study of the interaction of benproperine phosphate with eosin B and its analytical application

作　　者：冯素玲[1] 刘晶静[1] 樊静[1] 

机构地区：[1]河南师范大学化学与环境科学院,河南新乡453007

出　　处：《化学研究与应用》2008年第12期1551-1555,共5页Chemical Research and Application

基　　金：河南省自然科学基金资助项目(0511021200);河南省新乡市科技发展项目(07G052)

摘　　要：在pH为3．35的HAc-NaAc酸性介质中，曙红B（EB）与磷酸苯丙哌林（BPP）借助静电作用和疏水作用力发生相互作用形成EB—BPP离子缔合物，导致体系共振光散射（RLS）强度显著增强，并在364nm处出现最大散射峰。研究了体系的共振光散射光谱，吸收光谱及荧光光谱的特征，考察了实验条件及共存物质的影响。结果表明，在最佳的实验条件下，体系共振散射增强的强度与BPP的浓度在一定范围内呈良好的线性关系，其线性范围为0．041μg·mL^-1-2．00μg·mL^-1，检出限为14．31ng·mL^-1。据此，建立了一种高灵敏的定量测定活性组分BPP的方法。方法成功地用于药物、血清及尿样中BPP含量的测定。In HAc-NaAc buffer at pH 3.35 ,Eosin B （EB） can react with benproperine phosphate （BPP） to form an ion-association complex by electrostatic force and hydrophobic force, resulting in strong RLS signals at the maximum scattering wavelength of 364 rim. Spectral characteristics of resonance light scattering （ RLS）, absorption and fluorescence were studied. The affecting factors as well as the influence of coexisting substance have been investigated. The results indicate the enhanced RLS intensities are proportional to the concentration of BPP over the range of 0.041μg·mL^-1-2.00μg·mL^-1 ,with the detection limit of 14. 31 ng· mLl Base on this, A high sensitive method for the determination of BPP was established based on the RLS spectra, and could be applied to determination of BPP in pharmaceutical, serum and urine samples with satisfactory results.

关 键 词：共振光散射 曙红B 磷酸苯丙哌林 

分 类 号：O657.3[理学—分析化学]