高效液相色谱法测定人血浆中非那雄胺的浓度  

Determination of Finasteride in Human Plasma by HPLC

作　　者：余莲[1] 

机构地区：[1]重庆市第五人民医院,重庆400062

出　　处：《中国药业》2008年第24期19-20,共2页China Pharmaceuticals

摘　　要：目的建立测定人血浆中非那雄胺浓度的高效液相色谱(HPLC)法。方法以乙腈-10mmol/L磷酸二氢钾(40∶60)为流动相,炔雌醇为内标,血浆样品经乙酸乙酯萃取。结果非那雄胺质量浓度线性范围是9.44～944μg/L,平均相对回收率为94.5%～98.9%,日内和日间RSD均小于10%,非那雄胺的最低定量限为5μg/L。结论该方法快速、准确、灵敏,可用于非那雄胺的药代动力学研究。Objective To establish a HPLC method to detect the concentration of finasteride in human plasma. Methods A mixture of 10 mol/L potassium dihydrogen phosphate solution and acetonitrile（60 ： 40） was used as the mobile phase. Ethinylestradiol was used as the internal standard. The plasma samples were pretreated by ethyl acetate, and analyzed by HPLC. Results The range of linearity was 9.44- 944 μg/L （r =0. 999 5）. Both the within- day and between- day RSD were less than 10%. The average recovery rate was 94. 5% -98.9%. The minimum detective limit was 5 μg/L. Conclusion This method is accurate, stable and sensitive. It can be applied in the clinical phar- macokinetic study.

关 键 词：非那雄胺 高效液相色谱法 血浆 

分 类 号：R969.1[医药卫生—药理学] R983[医药卫生—药学]