PCR法快速检测阳性血培养瓶中葡萄球菌属  

Rapid Identification of Staphylococci in Positive Blood Culture Bottles by PCR

作　　者：周义正[1] 李向阳[2] 邱小燕[1] 付千均[1] 杨锦红[2] 

机构地区：[1]荆州市中心医院检验科,湖北荆州434020 [2]温州医学院附属第二医院检验科,浙江温州325027

出　　处：《中华医院感染学杂志》2008年第12期1798-1800,共3页Chinese Journal of Nosocomiology

摘　　要：目的建立采用聚合酶链反应（PCR）技术从阳性血培养瓶中检测葡萄球菌属的方法。方法以493例阳性血培养瓶中的细菌为检测对象，采用盐酸胍-苯甲醇法提取细菌DNA，然后以PCR扩增16S rRNA、ssa和mecA基因鉴定葡萄球菌属及其对甲氧西林的耐药性，并将检测结果与传统方法相比较。结果PCR法检测阳性血培养瓶中葡萄球菌属的时间约为4h。其较传统方法的24～48h明显缩短；以传统方法为金标准，其检测葡萄球菌属的灵敏度和特异度达98．6％和100．0％，此外PCR法检测耐甲氧西林葡萄球菌较传统方法也具有明显优势。结论该方法灵敏度高，特异性强，检测方便快速；适合从阳性血培养瓶中检测葡萄球菌属。OBJECTIVE To establish a method of polymerase chain reaction（PCR） for detecting staphylococci in positive blood culture bottles. METHODS Genomic DNA in 493 positive blood culture bottles was extracted by guanidine hydrochloride and benzenemethanol, then genes 16S rRNA, ssa and mecA were amplified by PCR to identify staphylococci. Finally, the results of PCR were compared with that of traditional method. RESULTS To compare with traditional method, as the golden standard the sensitivity and specificity of PCR method were 98.6 and 100. 0%, respectively, the detection could be finished in four hours. Method of PCR was better than traditional method in detecting meticillin-resistant staphylococci. CONCLUSIONS The PCR-based assay is simple, rapid, sensitive and specific, it can be used to detect staphylococci in positive blood culture bottles.

关 键 词：葡萄球菌属 聚合酶链反应 甲氧西林 耐药性 

分 类 号：R378.11[医药卫生—病原生物学]