人工加载对悬吊大鼠骨组织形态计量学影响的实验研究  

Influence of Artificial Loading to Tibia Histomorphometry of Suspended Rats

作　　者：刘易军[1] 孟广伟[2] 宫赫[2] 朱东[1] 朱伟民[2] 王静[1] 

机构地区：[1]吉林大学第一医院,长春130021 [2]吉林大学机械科学与工程学院,长春130022

出　　处：《中国生物医学工程学报》2008年第6期838-841,共4页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(10372034)

摘　　要：为探讨力负荷对废用性骨质疏松的影响,针对人工加载对悬吊去负荷SD大鼠胫骨松质骨组织计量学的影响进行了研究。选取9月龄清洁级雌性SD大鼠21只,分为对照组、悬吊组、悬吊加载组,采用自行研制的动态活体加载装置每日对悬吊加载组大鼠加载15min,频率选取5Hz,外部载荷选取3N。4周后处死大鼠,取左侧胫骨,采用显微CT扫描标本,进行胫骨组织计量学测定分析。结果表明悬吊组大鼠松质骨组织形态计量学参数与对照组及悬吊加载组相比显著改变;对照组与悬吊加载组松质骨组织形态计量学参数无显著差异。说明人工加载能显著改善悬吊大鼠松质骨形态计量学参数。The effects of artificial loading on the bone histomorphometry was investigated to evaluate the influence of loading to disuse osteoporosis. Twenty-one female SD rats were divided randomly into 3 groups： control, suspended, suspend ＆ loading. The suspend ＆ loading group was loaded in self-made device by 3 N and 5Hz for 15 minutes per day. 4 weeks later, bone histomorphometry of tibia were observed by micro CT. Results demonstrated that the bone histomorphometry parameters of cancellous bone of suspended group significantly differed from the control group and the suspend ＆ loading group （ P 〈 0.05）, while there were no significant difference between the control group and suspend ＆ loading group （ P 〉 0.05 ）. Artificial loading could improve the cancellous bone histomorphometry parameter of suspend rats significantily.

关 键 词：骨质疏松 松质骨 悬吊 骨组织形态计量学 显微CT 

分 类 号：R336[医药卫生—人体生理学]