成都地区肥胖患者GNB3基因825C／T多态性研究  

Analysis of the GNB3 gene 825C/T polymorphism in non-obese and obese Chinese

作　　者：王晓苏[1] 白怀[1] 范平[1] 刘瑞[2] 刘宇[3] 刘秉文[3] 

机构地区：[1]四川大学华西第二医院实验中心,成都610041 [2]四川大学华西第二医院人类疾病相关多肽研究室 ,成都610041 [3]华西基础医学与法医学院生物化学与分子生物学教研室

出　　处：《中华医学遗传学杂志》2008年第6期670-674,共5页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金（39770322）;四川大学华西医院科研启动基金

摘　　要：目的研究G蛋白郎亚单位（G-protein β3 subunit，GNB3）基因825C／T多态性是否与中国人肥胖有关联，为探讨肥胖的分子遗传基础提供依据。方法应用聚合酶链反应一限制性片段长度多态性分析法，对成都地区270名非肥胖者及129例肥胖患者GNB3基因825C／T多态性位点进行分析，采用酶法和单向免疫扩散法对血脂和载脂蛋白水平进行测定。结果GNB3基因825C／T位点C、T等位基因的频率在肥胖组为0．531、0．469，在非肥胖组为0．528、0．472，两组间等位基因的频率差异无统计学意义（P〉0．05）。中国人825C／T位点T等位基因频率（0．471，合并组）较德国白人的0．319显著增高（P〈0．01），而低于非洲黑人的0．788（P〈0．01），与日本人的0．487相近（P〉0．05）。825C／T位点在非肥胖组TT基因型携带者血清甘油三酯水平高于CT基因型者（P〈0．05）；在肥胖组cc基因型携带者血清高密度脂蛋白胆固醇水平较CT基因型者降低（P〈0．05）。进一步按性别分层后，这种差异仅在相应各组的男性、女性亚组存在；此外，非肥胖男性TT型者、女性CC型者血清高密度脂蛋白胆固醇、载脂蛋白AⅠ水平分别低于和高于相应亚组cT型者（P〈0．05），肥胖男性亚组TT型者血清载脂蛋白AⅠ水平高于CC型者（P〈0．05）。结论GNB3基因825C／T多态性与中国成都地区汉族人肥胖无关联，但与血清甘油三脂、高密度脂蛋白胆固醇和载脂蛋白AⅠ水平含量有关，且具有性别差异存在。Objective To investigate the G-protein β3 subanit （GNB3） gene 825C/T polymorphism and its rela- tionship to obesity in Chinese population. Methods Three hundred and ninety nine subjects （270 non-obese and 129 obese individuals） from a population of Chinese Han nationality in Chengdu area were studied using PCR-restrietion fragment length polymorphisms （PCR-RFLPs）. Serum hpids were measured by enzymatic kits and apolipoproteins AⅠ, A Ⅱ, B100, CⅡ, cⅢ and E were measured by R1D kits. Results The frequencies of C and T alleles at the 825C/T site in obese and non-obese groups were 0.531 and 0.469, and 0.528 and 0.472, respectively. It showed no significant difference in both genotypes and allele frequencies between the non-obese and obese groups （P 〉 0.05）. The frequency ofT allele at 825C/T site in GNB3 gene in the population （0.471） was significantly higher than that of German white （0.319）, lower than that of African black （0. 788）, and similar to that of Japanese （0.487）. In the non-obese group, subjects with genotype Tr had higher serum triglyceride（TG） concentrations than those with genotype CT （ P 〈 0.05）. In the obese group, subjects with genotype CC had lower serum high-density lipoprotein cholesterol（HDL-C） levels than those with CT genotype （ P 〈 0.05）. Similar results were only observed in non-obese male and obese female subgroups, respectively, when male and female subgroups were further separated in the two groups. In addition, non-obese males with genotype TT and obese females with genotype CC had lower HDL-C and higher apoAⅠ levels than those with geno- type CT, respectively. Obese males with genotype TT had higher apoA I levels than those with genotype CC. Conclu- sion The 825C/T polymorphism in the GNB3 gene was not associated with obesity in Chinese Han population of Chengdu area. However, it may be associated with serum TG, HDL-C and apoAⅠ levels, with some gender-specific effect, in this population.

关 键 词：肥胖 GNB3基因 遗传多态性 

分 类 号：R686[医药卫生—骨科学]