MCP-1在大鼠坐骨神经损伤中作用的实验研究  

An experimental study of the role of MCP-1 in a rat sciatic nerve injury model

作　　者：聂铭博[1] 康皓[2] 邢丹谋[1] 彭正人[1] 洪光祥[2] 

机构地区：[1]武汉市普爱医院手外科,430033 [2]华中科技大学同济医学院附属协和医院手外科,武汉

出　　处：《中华手外科杂志》2008年第6期336-339,I0005,共5页Chinese Journal of Hand Surgery

基　　金：湖北省自然科学基金项目（2004ABA192）

摘　　要：目的研究单核细胞趋化蛋白-1（MCP-1）在大鼠坐骨神经损伤后在神经组织中的表达以及抑制其作用对损伤的坐骨神经华勒变性过程的影响。方法96只雄性SD大鼠随机分为4组A、B、C、D（n=24）。另取42只随机分为2组E、F组（n=21），于右大腿后部中段切断坐骨神经，A、B组用聚乙烯软管套接固定神经远端于充满MCP-1抗体的微渗透泵直到取材，近端返折固定于临近肌肉。C、D、E、F组神经切断后远端旷置，近端处理同A组。A、B组于0h、24h、3d、7d（n=6）分别作光镜和电镜观察远端神经轴突和髓鞘形态变化，C、D组分别为其对照。E、F组在损伤后0h、6h、12h、24h、3d．7d、14d（n=3）取坐骨神经远端，E组实时荧光定量RT—PCR测定MCP-1mRNA表达，F组Western Blot测定MCP-1蛋白含量。结果神经损伤后，在24h和14d时E组MCP-1mRNA和F组MCP-1蛋白水平达到高峰，于24h时最高。光镜和电镜下各实验组髓鞘厚度均高于各自对照组。结论MCP-1在大鼠坐骨神经切割伤后在时间上存在分泌高峰，在神经损伤后远端华勒变性过程中起促进作用。Objective To study the expression of monocyte chemoattractam protein-1 （ MCP-1 ） in nerve tissue post murine ischiadic nerve damage and the impact of the inhibition on the impaired ischiadic nerve Wallerian degeneration process. Methods Ninety-six male SD rats were randomly divided into 4 groups ： A, B, G, D （ n = 24 ）, another 42 mtswere randomly divided into 2 groups ： E, F group （ n = 21 ）, ischiadic nerves were cut off in the posterior intermediate piece of the right thigh, in A, B groups, the nerve distal end was frxed by polythene vinyl hose sleeve joints and put into a MCP-1 Ab-flooded nficm-osmotic pump until use, the proximal end was back-folded fixed in the adjacent muscles. In C,D,E,F groups, the distal end of the nerves were put in the open post nerve disjunction, the disposal of the proximate is the same as A group. The morphologic changes of distant nerve axis and medullary sheath were observed with a light microscope and an electron microscope at 0 h,24 h,3 d,7 d in A,B greups（n =6）, and C,D groups were taken as control respectively. MCP-1 mRNA expression in E group was measured with real time fluorescent quantitation RT-PCR and MCP-1 protein level in F group was measured with Western Blot at 0 h,6 h, 12 h, 24 h,3 d,7 d, 14 d（n = 3）, post damage by taking ischiadic nerve distal end. Results The protein levels of MCP-1 mRNA in E group and MCP-1 in F group reached the peak at 24 h and 14 d post nerve damage, and were highest at 24 h. The medullary sheath thickness in each experimental group was higher than their respective control under light microscope and electron microscope. Conclusion MCP-1 presents a time- dependent secretion peak post murine ischiadic nerve concis and facilitates retm-distal end Wallerian degeneration process post nerve damage.

关 键 词：坐骨神经 髓鞘 单核细胞趋化蛋白-1 华勒变性 

分 类 号：R686[医药卫生—骨科学]