“逆针灸”干预对去卵巢大鼠下丘脑不同核团ER-α和ER-αmRNA表达水平的影响  

Experimental study on changes of expressions of ER-a and ER-αmRNA in different hypothalamic nuclei and the effect of"preventive acupuncture and moxibustion"in ovariectomy rats

作　　者：李晓泓[1] 郑玲[1] 解秸萍[1] 张露芬[1] 翟景慧[1] 周军[2] 田阳春[1] 周登芳[1] 何玉伟[1] 曲黎敏[3] 

机构地区：[1]北京中医药大学针灸学院,北京100029 [2]安徽中医学院第一附属医院 [3]北京中医药大学基础医学院

出　　处：《中国针灸》2008年第12期918-922,共5页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金项目:90209026;北京市自然科学基金项目:7052037

摘　　要：目的:探索"逆针灸"对去卵巢大鼠下丘脑功能的调节机制。方法:将3.5月龄SD雌性大鼠30只,随机分为正常组、假手术组、去卵巢模型组、逆针组、逆灸组。逆灸组与逆针组分别预先艾灸、针刺1个月后,与去卵巢模型组、假手术组同时造模。采用免疫组化和原位杂交方法,观察各组大鼠下丘脑室旁核、视上核、弓状核雌激素受体-α(ER-α)和基因表达水平的变化。结果:大鼠去卵巢后下丘脑室旁核ER-α及ER-αmRNA,视上核ER-αmRNA表达均明显升高(P<0.01),视上核、弓状核ER-α表达明显降低(P<0.01);与去卵巢模型组比较预先进行"逆针灸"处理后,大鼠下丘脑室旁核ER-αmRNA表达明显降低(P<0.05),ER-α无明显变化(P>0.05),视上核、弓状核ER-α表达明显升高(P<0.01,P<0.05),视上核ER-αmRNA无明显变化(P>0.05)。结论:"逆针灸""关元"穴能够影响去卵巢更年期大鼠下丘脑不同核团ER-α和ER-αmRNA表达水平的变化,可能是其调节下丘脑功能活动的途径之一。Objective To explore the mechanism of ＂preventive acupuncture and moxibustion＂ for regulating hypothalamic function in ovariectomy rats. Methods Thirty female SD rats, aged 3.5 months, were randomly divided into normal group, sham operation group, ovariectomy model group, preventive acupuncture group and preventive moxibustion group. After ＂preventive acupuncture and moxibustion＂ were previously given to the preventive acupuncture group and the preventive moxibustion group for one month, respectively. At the same time, ovarietomy was made in the preventive acupuncture group, the preventive moxibustion group and the ovariectomy model group.For the sham operation group, onlya little fat around the ovary was removed without ovariectomy, and nothing was not applied to the normal group. Immunohistochemical and in situs hybridization methods were used to investigate changes of expressions of ER-α and ER-α mRNA in paraventricular nucleus, supraoptic nucleus and arcuate nucleus;Results In the model group the expressions of ER-α and ER-α mRNA in paraventricular nucleus and the expression of ER-α mRNA in supraoptic nucleus increased significantly （P〈0.01）, but the expression of ER-α dropped significantly in both supraoptic nucleus and areuate nucleus （P〈0.01）. After preconditioning with acupuncture or moxibustion, the expression of ER-α mRNA in paraventricular nucleus significantly reduced （P〈0.05）, and the expression of ER-α had no significant change （P〉0. 05） ;the expression of ER-α in supraoptic nucleus and arcuate nucleus significantly increased （P〈0. 01 or P.〈0.05）, and the expression 0f ER-α mRNA in paraventricular nucleus had no significant change （P〉0. 05）. Conclusion ＂Preventive acupuncture and moxibustion＂ at ＂Guanyuan＂ （CV 4） have an effect on the expressions of ER-α and ER-α mRNA in different hypothalamic nuclei in ovariectomy rats, which possibly is one of the ways for modulating the hypothalamic activity.

关 键 词：逆针灸 大鼠 Sprague-Dawley 卵巢切除术 下丘脑 下丘脑室旁核 雌激素受体alpha 

分 类 号：R246.3[医药卫生—针灸推拿学]