胎猪皮肤前体组织异种移植研究  

Study of xenotransplantation of fetal pig skin precursor tissue

作　　者：黄正根[1] 吴军[1] 罗高兴[1] 贺伟峰[1] 甘成军[1] 袁顺宗[1] 贾雄飞[1] 谭江琳[1] 王晓娟[1] 葛良鹏[2] 魏泓[2] 

机构地区：[1]第三军医大学西南医院全军烧伤研究所,创伤、烧伤与复合伤国家重点实验室,重庆400038 [2]第三军医大学基础医学部实验动物学教研室

出　　处：《中华烧伤杂志》2008年第6期437-440,共4页Chinese Journal of Burns

基　　金：国家高技术研究发展计划（2006AA02A121）

摘　　要：目的系统筛选猪胚胎皮肤前体组织异种移植的最佳妊娠时间窗，了解其创面修复能力。方法取胎龄35、42、56、70d的胎猪皮肤前体组织，制成微粒后移植于BABL／c裸鼠背部创面，以整形患者术后剩余皮肤或者成年猪皮覆盖。观察术后移植物生长发育特性，并于移植后6、12周取材，用组织学方法观察其形态结构及成瘤性。结果胎猪皮肤前体组织移植后具有如下特点：（1）能成活并继续生长发育，微粒可融合成片；胎龄35、42、56、70d的前体组织移植后12周，新生组织面积分别为（18±8）、（47±6）、（31±12）、（20±8）mm^2，其中胎龄42d的组织与其余三者比较，差异有统计学意义（P〈0．05）。（2）新生（猪）皮肤组织具有表皮层和真皮层，真皮乳头明显。（3）能生长成为具有毛发、皮脂腺及汗腺等皮肤附属器的“完整”皮肤，且表皮层有黑素细胞。（4）胎龄56、70d的胎猪皮肤前体组织移植后未出现畸胎瘤。结论胎龄56d的胎猪皮肤前体组织可用于异种移植修复皮肤创面。Objective To select the optimal pregnancy time window of embryonic pig skin precursor tissue for xenotransplantation and study its ability in wound repair. Methods Skin precursor tissues were obtained from pig fetus of fetal age of 35,42,56,70 days, and were minced into microskin and transplanted to dorsal wounds of BABL/c nude mice, then they were covered with residual skin after plastic surgery of patients or adult pig skin（white）. The characteristics of growth and development were observed after transplantation. Pathological examination was performed on 6 and 12 post operation weeks respectively to observe the tissue structure and tumorigenicity. Results Skin precursor tissues from fetal pig survived and developed after transplantation, and the microskin fused. New tissue area from skin precursor tissues with fetal age of 42 days was （47±6）mm^2, which was higher than that of 35 days（18±8 mm^2） ,56 days （31±12 mm^2） ,70 days（20±8 mm^2, P 〈 0.05 ）. The skin precursor developed into ＂ intact skin＂ with hair, sebaceous glands and sweat glands, and melanocytes were also detected in epidermis. The newly-grown skin tissue included epidermal and dermal layer, and obvious dermal papillae. Terotoma was not found after transplantation in skin precursor tissue with fetal age of 56 ,70 days. Conclusion Fetal pig skin precursor tissue with fetal age of 56 days can be used to repair wound as xenotransplantion.

关 键 词：移植 异种 胚胎 皮肤前体组织 创面修复 

分 类 号：R622[医药卫生—整形外科]