实验性衰老大鼠海马和大脑脑源性神经营养因子表达的变化  

Study on the expression of BDNF in hippocampus and cerebrum in D-galactose-induced aging rats

作　　者：宋士军[1] 李怡秋 李芳芳 马骏骥 张若楠[1] 

机构地区：[1]河北医科大学生理教研室,河北石家庄050017 [2]生物化学和分子生物学教研室

出　　处：《中国老年学杂志》2008年第23期2310-2312,共3页Chinese Journal of Gerontology

基　　金：河北省科学技术研究与发展指导计划支持(No.05276454)

摘　　要：目的探讨实验性衰老大鼠大脑和海马脑源性神经营养因子(BDNF)表达的变化及何首乌的作用。方法给大鼠皮下注射大剂量D-半乳糖0.125g.kg-1.d-1,连续注射60d,通过Morris水迷宫测定大鼠的空间学习记忆能力,用RT-PCR和Western印迹法测定大脑皮层和海马BDNFmRNA和蛋白质水平表达的变化。结果与正常对照组比较,D-半乳糖组大鼠逃避潜伏期延长,表明模型大鼠的空间学习记忆能力降低,大脑和海马CA1/CA2组织BDNF mRNA和proBDNF表达量降低,而大脑和海马CA1/CA2组织BDNF表达量没有变化。结论D-半乳糖可引起大鼠的学习记忆能力下降,这可能与大脑和海马proBDNF含量降低有关。何首乌可以改善大鼠的空间学习能力,但是不改变BDNF mRNA的表达和proBDNF蛋白质的含量。Objective To explore the influence of Radix Polygoni Multiflori （RPM） on brain-derived neurotrophic factor （BDNF） expression of hippocampus and brain in the senile rat induced by D-galactose （D-gal）. Methods 36 rats were randomly divided into control, senile model and RPM groups. The model and RPM group were injected with D-gal 0. 125 g · kg^-1 · d^-1 by subcutaneous injection continuously for 60 days, while control group were administered with the same dose of normal saline per day. The ability of learning and memory of rats were measured in Morris water maze, the amount of mRNA of BDNF was measured by RT-PCR and the amount of protein of BDNF was measured by Western blot. Results The escape latency of model group was longer than that of control group （P 〈 0. 05）, and there was no difference between RPM group and control group （P 〉 0.05 ）. There was no difference of BDNF mRNA in hippocampus （P 〉 0. 05 ） and in brain （P 〉 0. 05） between RPM group and model group, but those of model group were lower than control group in hippocampus （P 〈 0. 05） and in brain （P 〈 0. 05 ）. In the case of pro-BDNF, there was no difference between RPM group and model group in hippocampus （ P 〉 0. 05 ） and brain （ P 〉 0. 05 ）, but those of model group were lower than those of control group in hippocampus （ P 〈 0. 05 ） and in brain（P 〈 0. 05）. Conclusions D-gal could induce the defect of learning and memory in rats, being related to the decrease of proBDNF in hippocampus and brain. RPM could delay the deterioration of learning and memory induced by D-gal, however, it could not change the expression of BDNF mRNA and the level of proBDNF.

关 键 词：学习记忆 大鼠 D-半乳糖 衰老 脑源性神经营养因子 何首乌 

分 类 号：R338.64[医药卫生—人体生理学] R339.38[医药卫生—基础医学]