白细胞介素-2基因多态性与慢性乙型肝炎的相关性  

Correlation of polymorphisms of interleukin-2 gene and HBV DNA copies in patients with chronic hepatitis B

作　　者：韦叶生[1] 蓝艳[2] 袁锡华[1] 唐任光[1] 黄重敏[3] 王春芳[1] 

机构地区：[1]广西百色市右江民族医学院附属医院临床检验中心,533000 [2]广西百色市右江民族医学院附属医院皮肤科,533000 [3]广西百色市右江民族医学院附属医院传染科,533000

出　　处：《中华肝脏病杂志》2008年第12期889-892,共4页Chinese Journal of Hepatology

摘　　要：目的探讨白细胞介素-2（IL-2）基因多态性与慢性乙型肝炎的相关性，以及对HBVDNA水平的影响。方法应用聚合酶链反应-限制性片段长度多态性分析和DNA测序的方法检测155例慢性乙型肝炎患者和170名健康对照者的IL-2基因-385T／G、＋114T／G单核苷酸多态性位点基因型，血清HBVDNA测定采用荧光定量PCR技术。结果IL-2基因＋114T／G多态性在慢性乙型肝炎组和正常人群中的频率分布差异无统计学意义。IL-2基因-385T／G多态性在两组人群中的频率分布差异有统计学意义，Χ^2=7．377，JD〈0．05。等位基因频率的相对风险分析发现，G等位基因携带者患慢性乙型肝炎的风险是T等位基因的1．490倍（OR=1．490，95％CI：1．085～2．046）；进一步比较慢性乙型肝炎患者IL-2基因多态性与HBVDNA复制的关系，发现高水平HBVDNA（≥1×10^3拷贝／ml）组385G等位基因携带者分布频率明显高于低水平HBVDNA组（〈1×10^3拷贝／ml），Χ^2=6．051，P=0．014，差异有统计学意义。结论IL-2基因-385T／G多态性可能与HBV具有相关性，其中G等位基因可能是慢性乙型肝炎的遗传易感基因，携带G等位基因的个体可能更利于HBVDNA的复制。Objectives To investigate the relationships ofpolymorphisms ofinterleukin-2 （IL-2） gene to the susceptibility of chronic hepatitis B, and to analyze the frequencies of IL-2 gene polymorphisms and the HBV-DNA copies in patients with chronic hepatitis B. Methods Two single nucleotide polymorphisms of IL-2 gene promoter -385T/G and ＋114T/G at exon one were analyzed in 155 patients with chronic hepatitis B and in 170 age- and sex-matched controls from a Chinese population. Polymerase chain reaction- restriction fragment length polymorphism （PCR-RFLP） strategy and DNA sequencing were used, and the serum HBV DNA in these patients was determined by real-time PCR. Results There were no differences in the distributions of IL-2 gene ＋114T/G polymorphism in the patient group and the controls, but their IL-2 gene -385T/G polymorphisms were significantly different （P 〈 0.05）. The relative risk of being chronic hepa- titis B victims with T allele was 1.490 times of those with G allele （OR = 1.490, 95% CI： 1.085-2.046）. The association between the genotype of IL-2 gene polymorphisms and HBV-DNA copies showed that the frequency of-385G allele carriers in high HBV-DNA copy group was closer than that in low HBV-DNA copy group （ Χ^2 = 6.051, P = 0.014）. Conclusions IL-2 gene -385T/G polymorphism is associated with chronic hepatitis B and G allele is an important genetic susceptibility gene for chronic hepatitis B. In IL-2 gene -385G allele carriers the risk of increasing HBV-DNA copies may be related to the pathogenesis of chronic hepatitis B.

关 键 词：肝炎 乙型 慢性 肝炎病毒 乙型 白细胞介素-2 多态现象(遗传学) 

分 类 号：R512.62[医药卫生—内科学] R730.53[医药卫生—临床医学]